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Lactobacillus plantarum and application thereof in pig feed

A technology for Lactobacillus plantarum and sow feed, applied in the field of Lactobacillus plantarum, can solve the problems of low reproductive performance, sub-health of sow groups, low immune function, etc., so as to enhance disease resistance, improve growth performance, and enhance immunity. force effect

Active Publication Date: 2021-09-03
山东玖瑞农业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The second purpose of the present invention is to provide a Chinese herbal medicine fermented feed suitable for sows in view of the problems of sub-health, low immune function and low reproductive performance of sows caused by excessive use of chemically synthesized drugs in the current pig industry Additive; the provided feed additive is prepared by using the above-mentioned fermented Chinese herbal medicine raw material of Lactobacillus plantarum HS-1

Method used

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  • Lactobacillus plantarum and application thereof in pig feed
  • Lactobacillus plantarum and application thereof in pig feed
  • Lactobacillus plantarum and application thereof in pig feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: screening Lactobacillus plantarum HS-1

[0028] The method for obtaining the Lactobacillus plantarum HS-1 is as follows:

[0029] (1) Screening and separation and purification of strains

[0030] To screen acid-producing bacteria from rotting food waste, take 1 g of food waste sample, add 9 mL of sterile saline, and vortex for 10 minutes to make an initial suspension of 1:10, and then gradually dilute the sample with normal saline 10 times. Choose 3 to 5 appropriate dilutions, take 1mL of the dilution in a sterilized plate, pour it into MRS medium (add 0.2% calcium carbonate) cooled to about 40 degrees, mix well, and wait for the medium to cool and solidify, Place it upside down in a 37-degree constant temperature incubator for 24-72 hours.

[0031] Select 4 single colonies with large calcium-dissolving circles on the plate, and separate them by streaking on the MRS medium several times to obtain pure cultures, which are named HS-1, HS-2, HS-3, and HS-4 ...

Embodiment 2

[0044] A feed additive suitable for sows is prepared by fermentation of Lactobacillus plantarum L.plantarum HS-1, and the specific steps are as follows:

[0045] (1) Select the raw materials of various medicinal materials of Radix Astragali, Atractylodes macrocephala, Radix Paeoniae Alba, Purslane, and Poria cocos respectively to remove contained impurities;

[0046] (2) Various medicinal materials are pulverized respectively, so that the pulverized particles can pass through a 20-mesh sieve;

[0047] (3) Weigh Chinese herbal medicines comprising the following qualities: Astragalus 30g, Atractylodes macrocephala 25g, Radix Paeoniae Alba 20g, Purslane 10g, Poria cocos 15g, mix well;

[0048] (4) Add 2% molasses as a carbon source, add 3% of Lactobacillus plantarum liquid (OD 6-7) in the logarithmic phase, mix evenly, adjust the moisture to 40%, and mix evenly.

[0049](5) Put the mixed material into a breathing fermentation bag, seal and ferment at 37° C. for 3 days, and obtai...

Embodiment 3

[0052] A feed additive suitable for sows is prepared by fermentation of Lactobacillus plantarum L.plantarum HS-1, and the specific steps are as follows:

[0053] (1) Select the raw materials of various medicinal materials of Radix Astragali, Atractylodes macrocephala, Radix Paeoniae Alba, Purslane, and Poria cocos respectively to remove contained impurities;

[0054] (2) Various medicinal materials are pulverized respectively, so that the pulverized particles can pass through a 20-mesh sieve;

[0055] (3) Take by weighing the Chinese herbal medicine that comprises following quality: Astragalus membranaceus 20g, Atractylodes macrocephala 30g, Radix Paeoniae Alba 20g, Purslane 15g, Poria cocos 15g, mix evenly;

[0056] (4) Add 2% molasses as a carbon source, add 5% of the logarithmic Lactobacillus plantarum liquid (OD 6-7), mix evenly, adjust the moisture to 45%, and mix evenly.

[0057] (5) Put the mixed material into a breathing fermentation bag, seal and ferment at 35° C. fo...

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Abstract

The invention provides lactobacillus plantarum HS-1, the preservation number of the lactobacillus plantarum HS-1 is CGMCC NO.21670. The lactobacillus plantarum HS-1 provided by the invention is used for preparing a traditional Chinese medicinal material feed additive. The lactobacillus plantarum HS-1 disclosed by the invention has high acid production capacity, can produce acid by fermenting Chinese herbal medicines serving as raw materials, inhibits the growth of other microorganisms, gives a special flavor to the fermented product, and improves the palatability. When the lactobacillus plantarum HS-1 is used for fermenting Chinese herbal medicine, the content of soluble polypeptide in Chinese herbal medicine raw materials can be increased, absorption and utilization of the Chinese herbal medicine in animal bodies are facilitated, plant cell structures of medicinal materials are broken through microbial fermentation, medicinal components wrapped by cellulose, hemicellulose and the like are fully released, and the absorption utilization speed of sows to medicine is increased, the medical components take effect faster and are obviously better than common traditional Chinese medicine powder and decoction.

Description

technical field [0001] The invention belongs to the technical field of probiotic screening, and in particular relates to a plant lactobacillus used for preparing a Chinese herbal feed additive for sows. Background technique [0002] With the development of large-scale pig farming in China, the number of breeding pigs on hand in pig farms is increasing year by year. However, in recent years, due to poor feeding management and abuse of various chemically synthesized drugs, hormones, antibiotics and other factors, the immune function of the sow population has generally declined, and the reproductive performance is not high. In addition, high-density captivity leads to insufficient exercise of sows, low immunity, weak spleen and stomach, insufficient Qi and blood, prone to tear spots, dead skin, constipation, repeated inflammation, no estrus, dystocia, anorexia, and lack of energy. The health status of the sow group also indirectly affects the survival rate and growth performan...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K10/30A23K10/33A23K10/12
CPCA23K50/30A23K10/30A23K10/33A23K10/12A23V2400/169Y02P60/87
Inventor 蔡冬梅刘进国刘向伟
Owner 山东玖瑞农业集团有限公司
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