Lactobacillus plantarum 360 with function of regulating intestinal flora and application of lactobacillus plantarum 360

A technology for regulating intestinal flora and Lactobacillus plantarum, applied to Lactobacillus plantarum 360 and application fields, can solve problems such as poor flora imbalance improvement, and achieve the effects of good application prospects, strong probiotic properties and fast growth.

Pending Publication Date: 2021-08-10
SICHUAN GAOFUJI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Lactobacillus plantarum, as a kind of probiotics, can improve intestinal mucosal barrier, regulate intestinal flora, reduce intestinal bacterial translocation, inhibit pathogenic microorganisms, improve intestinal mucosal immunity and inflammation, but existing strains still have improved bacteria. Therefore, it is very important to develop strains with better targeted regulation of intestinal function

Method used

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  • Lactobacillus plantarum 360 with function of regulating intestinal flora and application of lactobacillus plantarum 360
  • Lactobacillus plantarum 360 with function of regulating intestinal flora and application of lactobacillus plantarum 360
  • Lactobacillus plantarum 360 with function of regulating intestinal flora and application of lactobacillus plantarum 360

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Comparison scheme
Effect test

Embodiment 1

[0050] This example discloses the isolation, screening and molecular biological identification of Lactobacillus plantarum 360 of the present invention.

[0051] 1. Sample Collection

[0052] The samples were collected at the Xindu farmhouse in Chengdu.

[0053] 2. Strain Isolation

[0054] Cut the collected sample into pieces, weigh 1 g, and put it into 9 mL of sterile saline. After fully oscillating and mixing, 10-fold serial dilution was spread on MRS solid medium, and cultured at 37°C for 48h. Visually observe, pick single colonies of different shapes and sizes in the culture medium, streak and purify more than 4 times. It was initially identified as lactic acid bacteria by Gram staining and calcium dissolution method, and the purified strain was stored in 45% glycerol at -80°C for later use.

[0055] 3. Molecular biological identification

[0056] Molecular biological identification of strains: the purified strains were sent to the Culture Collection Center of the Sic...

Embodiment 2

[0058] This example discloses the morphology and bacteriostasis test of Lactobacillus plantarum 360 of the present invention.

[0059] 1. Morphological observation

[0060] Streak the purified strain on the MRS agar medium, culture it upside down at 37°C for 48 hours, observe the colony morphology of the strain, and the results are as follows: figure 1 As shown, the strain grows well on the MRS agar medium, and the colonies are round, medium in size, milky white, raised upwards, and the edges of the colonies are relatively neat and moist. Positive after Gram staining, morphology under microscope figure 2 , the morphology under the transmission electron microscope is shown in image 3 .

[0061] 2. Antibacterial test

[0062] Inhibition of the growth of Klebsiella DNL03, Staphylococcus aureus CMCC 26003, Bacillus cereus CMCC 63303, Streptococcus mutans CGMCC1.2499 and Clostridium perfringens ATCC13124:

[0063] Pour 10mL of water agar medium into a sterile plate, put it i...

Embodiment 3

[0066] This embodiment discloses the growth ability test of Lactobacillus plantarum 360.

[0067] Inoculate the Lactobacillus plantarum 360 strain into the MRS liquid medium at an inoculation amount of 5%, activate and cultivate at 37°C for 24 hours, and activate twice continuously; Inoculate into MRS liquid medium, mix evenly, and divide into sterile test tubes (18mm×180mm test tube) according to 8mL / branch; place the divided Lactobacillus plantarum 360 bacterial solution in a constant temperature incubator at 37°C and let it stand Cultivate, and take 3 test tubes to measure the absorbance value OD600 of the bacterial solution, and calculate the average value; at regular intervals, take 3 test tubes to measure the OD600 value of the 360 ​​bacterial solution, and calculate the average value; and draw the growth curve.

[0068] See the growth curve of Lactobacillus plantarum 360 strain Figure 5 ,from Figure 5 It can be seen from the figure that the strain grows rapidly and ...

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Abstract

The invention discloses lactobacillus plantarum 360 with a function of regulating intestinal flora, and belongs to the technical field of biology. The lactobacillus plantarum disclosed by the invention is preserved in the China Center for Type Culture Collection on June 5, 2015, and the preservation number is CCTCC NO: M 2015345. The lactobacillus plantarum 360 disclosed by the invention is applied to preparation of medicines or functional foods for regulating intestinal flora. The lactobacillus plantarum 360 disclosed by the invention has the advantages of fast growth, strong acid production capacity, gastric acid resistance and cholate resistance, has the probiotic characteristics of degrading nitrite, inhibiting pathogenic bacteria, regulating intestinal flora and the like, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Lactobacillus plantarum 360 with the function of regulating intestinal flora and its application. Background technique [0002] The trillions of microbes (mostly bacteria, but also viruses, fungi, and protozoa) that reside in the human gastrointestinal tract are collectively known as the gut flora. A large number of studies from animal models and humans have shown that gut flora plays an important role in many aspects of human health, including the relationship between gut flora and obesity, diabetes and other metabolic diseases such as Inflammatory bowel disease (IBD), class Rheumatoid arthritis (RA), functional constipation, and irritable bowel syndrome (IBS) are closely related to the occurrence and development of diseases (Li D, Gao C, Zhang F, et al. Seven facts and five initiatives for gut microbiome research. Protein Cell. 06 2020;11(6):391-400). In a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/00A23L33/135A23L2/39C12R1/25
CPCA61K35/747A61P1/00A23L33/135A23L2/39A23V2002/00A23V2400/169A23V2200/3204A23V2250/612A23V2250/5118A23V2250/51084A23V2250/5114A23V2250/61A23V2250/642A23V2250/284A23V2250/708A23V2250/264A23V2250/044
Inventor 蒲小平刘梅舒梨闾中平谢建将景晓青
Owner SICHUAN GAOFUJI BIOLOGICAL TECH
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