Lactobacillus paracasei strain and application thereof

A paracheese and lactobacillus technology, applied in the field of microorganisms, can solve the problems of variable and unpredictable effects of lactobacillus, and achieve the effects of inhibiting allergy and inflammation, excellent acid-producing ability, and regulating immune response.

Active Publication Date: 2015-09-09
I LAN FOODS IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Lactobacillus casei Daita strain is a kind of Lactobacillus used in the famous lactic acid bacteria beverage Yakult. Although it has certain acid production ability, imm

Method used

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  • Lactobacillus paracasei strain and application thereof
  • Lactobacillus paracasei strain and application thereof
  • Lactobacillus paracasei strain and application thereof

Examples

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Example Embodiment

[0042] Example 1: 16s rDNA and pheS sequence identification

[0043] V0151 was isolated from children’s feces in Taiwan. After being cultured in MRS medium at 37°C for one day, its genomic DNA was extracted, and 16s rDNA and pheS primers (as shown in Table 1) were used to perform Polymerase Chain Reaction on the genomic DNA. , PCR) to multiply specific DNA sequences, and use the National Center for Biotechnology Information (NCBI) database for strain comparison. The results are shown in Table 2 and Table 3.

[0044] Table 1. 16s rDNA and pheS primer sequences

[0045]

[0046] Table 2 shows the results of sequence comparison using 16S rDNA primers for propagation. The 16S rDNA sequence of V0151 is identical to the other two strains of Lactobacillus paracasei subsp. Paracasei (AM087710, AM087711), but it is similar to several other Lactobacillus strains. The strains have some sequence differences. Therefore, the isolate V0151 can be confirmed as Lactobacillus paracasei.

[0047] Sinc...

Example Embodiment

[0053] Example 2: Fermentation ability of Lactobacillus paracasei subspecies V0151

[0054] The API 50CHL rapid identification kit (bioMerieux, France) was used to test the fermentation ability of Lactobacillus paracasei subspecies V0151 on various carbohydrate substrates. The results are shown in Table 4.

[0055] Table 4. Fermentation ability of Lactobacillus paracasei subspecies V0151 on different carbohydrate substrates

[0056] Numbering Carbohydrate matrix result Numbering Carbohydrate matrix result 0 Control group -25 Horse leaf spirit iron citrate+ 1 glycerin -26 Salicin+ 2 Erythritol -27 D-cellobiose+ 3 D-arabinose -28 D-maltose+ 4 L-arabinose -29 D-lactose (source is cow)+ 5 D-ribose+30 D-Melibiose - 6 D-xylose -31 D-sucrose+ 7 L-xylose -32 D-trehalose+ 8 D-Ribitol+33 Inulin - 9 Methyl-β-D-xylopyranoside -34 D-melezitose - 10 D-galactose+35 D-Raffinose - 11 D-glucose+36 starch - 12 D-fructose+37 Glycogen - 13 D-Mannose+38 Xylitol - 14 L-Sorbitose+39 Almonds+ 15 L-rhamno...

Example Embodiment

[0058] Example 3: Acid production ability of Lactobacillus paracasei subspecies V0151

[0059] Lactobacillus paracasei subsp. paracasei V0151 was cultured in MRS medium, and the bacterial solution was taken out at 24, 36 and 72 hours for coating culture and counting. Take out one percent of the volume of the secondary activated bacteria solution, and incubate the culture solution based on skim milk at 37°C for 24 hours, 36 and 72 hours. Take out the culture solution after fermentation to test the pH value of the culture solution and perform acid titration In the experiment, the Daitian bacteria isolated from Yakult were taken as a control.

[0060] In the acid titration test, every 5 g of fermented culture broth was mixed with 20 ml of pure water, and titrated with 0.1N sodium hydroxide to calculate the acid production of the strain to be tested. The end point of the titration is set between pH 8.30 and 8.32, and the calculation formula for the acid production of lactic acid bacte...

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Abstract

The invention relates to the field of microbes, and particularly discloses a Lactobacillus paracasei strain and application thereof. The collection number of the Lactobacillus paracasei strain is CGMCC No.8903; and the Lactobacillus paracasei strain is separated from feces of children, and belongs to Lactobacillus paracasei subspecies. The strain has the advantages of high proliferation speed and excellent acid production capacity, has the capacities for secreting extracellular polysaccharides and resisting bile salt and gastric acid, is capable of adjusting immunoreaction and inhibiting allergy and inflammation, and is very suitable for preparing food, health-care products, medicines, food replenishers and other products.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a kind of Lactobacillus paracasei and its application. Background technique [0002] Currently, immune symptoms such as allergy, asthma, chronic rhinitis, atopic dermatitis or inflammatory symptoms are becoming more and more popular in developing or developed countries. The most commonly used treatment method is oral or smeared steroids, but its disadvantage is that it is prone to side effects such as making patients fat or increasing the infection rate, and even the risk of death. Many research groups are developing safer ways to relieve or prevent allergic and immune diseases with as few side effects as possible. [0003] It is generally known that Lactobacillus (Lactobacillus spp.) is a group of microorganisms that live in the body and benefit the health of the host. Its role in maintaining human health and regulating immune function has been widely recognized. Specific strains of th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A23L1/29A61P37/02A61P37/08A61P29/00
Inventor 洪维鍊杨政华陈俊江曹永梅蔡英杰
Owner I LAN FOODS IND
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