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Ecological tissue culture container for factorial culture

A factory-like, tissue-culturing technology, applied in the field of plant tissue culture or micropropagation, can solve problems such as lack of pollution prevention, achieve the effects of saving materials and manpower, reducing costs, and improving operational efficiency

Inactive Publication Date: 2004-01-07
刘思九 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because they are not specially constructed to prevent microorganisms from entering the container to prevent contamination
In addition to different uses, the above-mentioned patents each have two water storage structures, which are obviously different from the present invention
[0015] Some other relevant U.S. patents such as US4,908,315 US5,088,231 US5,199,588 US5,171,683 US5,212,906 and US5,525,505 etc. all have special devices or containers to protect the medium from contamination, but all these containers are closed culture , isolated from normal ecological conditions, the delicate nature of its tissue culture seedlings is still unavoidable

Method used

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  • Ecological tissue culture container for factorial culture
  • Ecological tissue culture container for factorial culture
  • Ecological tissue culture container for factorial culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077]Embodiment 1, front and rear movable type: this type is a combined structure of two parts inside and outside, and the inner part can move back and forth in the outer part to control the water supply. The outer part 10 of the container contains a plurality of water storage compartments 12 with a plurality of protrusions or plugs 14 on the inner walls of the compartments. But the most important feature of the ecological tissue culture container is that there is a ring of blocking structure 16 on the outer part, so as to prevent the powder from slipping and forming a gap at the part where the powder contacts the container wall, thereby blocking the intrusion of microorganisms. The blocking structure 16 is stepped in this type (FIG. 1). The step is wider at both ends of the container and narrower at both sides, so that the inner part or culture medium carrier 18 hanging on the step can be pushed back and forth without leakage (see FIG. 2 ). The water storage compartment 12 ...

Embodiment 2

[0084] Embodiment 2, up and down movable type: this type is a one-piece structure. It has a concave-convex bottom surface that can be pushed up and down. Its culture medium carrier 18 is not a single piece, but an integral part of the container (seeing Fig. 15 to Figure 19 ). The medium carrier 18 and its plurality of compartments 22 can be seen in plan view (FIG. 15). There is also an extension tube 20 for each compartment. There is a pipe 36 on the culture medium carrier, which needs to be sealed with common sealing material 37 after adding water. The section from line 16-16 shows more detail (Fig. 16), and all the components are inseparable as a whole.

[0085] The present embodiment also has many water storage compartments 12, and each compartment has an independent bottom surface and a partition wall not shared with adjacent rooms. Therefore, the water storage compartments are basically independent of each other, yet their centripetal ends are all covered. They are c...

Embodiment 3

[0088] Embodiment 3, solid water storage type: different from liquid water storage, this type uses solid water absorbing material 44 to absorb water storage liquid. Such materials as vermiculite, sand, perlite etc. They can absorb a considerable amount of water, and when the water is removed, their volume does not shrink much, and they will not sink due to the consumption of water.

[0089] The solid water storage type ecological tissue culture container is similar to the outer part of embodiment one. Below the stagnant structure, it is divided into several solid water storage compartments by compartment partition walls 23 . The above-mentioned solid water-absorbing material 44 is packed into and occupies most of the space of the water storage chamber. Medium 26 is placed on solid absorbent material (see Figure 22 and Figure 23 ), but not exceeding the height of the partition wall 23. In order to avoid excessive infiltration of medium components into the storage water, a ...

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Abstract

The present invention relates to a "ecological tissue culture container" suitable for factorial culture, and is characterized by that it possesses a circle of retarding structure and several culture medium separation chambers, and a layer of dried, hydrophobic and sterilized fine-sandy powder is spread on the explant and medium, said retarding structure protects the germ-free condition of the container instead of sealing mode so as to prevent microbe from invading into the container. The tissue cultured seeling can pass through the powder layer, grow up and extend from said container to weather normal ecological condition.

Description

technical field [0001] The invention belongs to plant tissue culture or micropropagation technology, more specifically it is an ecological method for industrialized tissue culture that allows tissue culture seedlings to grow out of the container and be exposed to normal ecological climate conditions to continue in vitro culture. Tissue culture container. Background technique [0002] Tissue culture seedling production is much more costly than traditional nursery production, thus severely limiting the development of the tissue culture industry. The main reason for the high cost is that the tissue culture seedlings grow too delicately in test tubes or other containers, and cannot adapt or even survive when transferred from test tubes or other containers to normal ecological conditions. In traditional tissue culture, in order to protect the medium from microbial contamination, the plant tissue and the medium are sealed in a container. Therefore, the responsibility for the tis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 刘思九徐宛南
Owner 刘思九
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