Full-automatic sheep blood PBMC separation system and method

Abstract

The invention discloses a full-automatic sheep blood PBMC (peripheral blood mononuclear cell) separation system and method. The full-automatic sheep blood PBMC separation system comprises an input module, a separation module, an output module, a pipeline module and a power module, wherein the pipeline module comprises an input pipe, a peristaltic pipe and an output pipe; the head end of the input pipe is provided with a filter membrane structure, and the tail end of the input pipe and the head end of the output pipe both communicate with the head end of the peristaltic pipe; and the input end and the output end of the separation module both communicate with the tail end of the peristaltic pipe, and the power module is arranged on the peristaltic pipe and used for driving liquid in the peristaltic pipe to flow. The separation process is full-automatic, the consistency of the production environment, the raw materials and the production process can ensure that each batch of cells are prepared under the same standard condition, and the stability and uniformity of the cell quality and the repeatability of experimental results are ensured. The problem that the peripheral blood mononuclear cells need to be extracted and prepared on a large scale in scientific research and new drug research and development animal experiments is solved, traditional manual operation is changed into automatic operation through intelligent equipment, efficiency is improved, and time of scientific research personnel is saved.

Description

technical field [0001] The invention relates to the technical field of PBMC separation, in particular to a fully automatic sheep blood PBMC separation system and method. Background technique [0002] PBMC (Peripheral blood mononuclear cel) is a peripheral blood mononuclear cell, that is, a cell with a single nucleus in peripheral blood, including lymphocytes, monocytes, dendritic cells and a small number of other cells. Through the isolation, screening and cultivation of PBMC, a large number of immune cells can be obtained for corresponding immunotherapy. [0003] Most of the traditional PBMC separation methods are manually separated by scientific researchers using centrifuge tubes and centrifuges in clean laboratories, which requires high-level operating technicians, takes a lot of time, and requires a large laboratory space. It has the following determinations: [0004] (1) The efficiency is low, and it cannot achieve high efficiency in the true sense; [0005] (2) The ...

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Problems solved by technology

[0003] Most of the traditional PBMC separation methods are manually separated by scientific researchers using centrifuge tubes and centrifuges in clean laboratories, which requires high-level operating technicians, takes a lot of time, and requires a large laboratory space. It has the following determinations:

[0004] (1) Low efficiency, unable to achieve real high efficiency;

[0005] (2) The preparation process is affected by human subjective experience, the quality of the final product is uneven, and the quality stability cannot be guaranteed, which brings difficulties to the selection of subsequent scientific parameters;

[0006] (3) Manual intervention brings two major problems: various pollution risks; problems caused by too long time; the safety of elements such as quantity, activity, and purity cannot be guaranteed

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