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Efficient eucommia ulmoides regeneration method based on LED light quality regulation and control

A high-efficiency regeneration and light quality technology, applied in the biological field, can solve the problems of limiting the preservation and large-scale propagation of eucommia germplasm, failing to meet the large-scale planting of eucommia, and the low efficiency of adventitious bud proliferation, achieving short disinfection time and low toxicity , the effect of reducing energy consumption costs

Active Publication Date: 2021-09-14
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are reports on the propagation of Eucommia ulmoides using plant tissue culture technology, but they mainly focus on the seedlings as the source of explants, and there are serious pollution, adventitious buds Problems such as low multiplication efficiency and low rooting efficiency seriously limit the germplasm preservation and large-scale propagation of Eucommia ulmoides improved varieties
[0008]In summary, the traditional seedling cultivation process of Eucommia is time-consuming and laborious, limited by seasons and has low regeneration efficiency, which cannot meet the needs of large-scale planting of Eucommia. Reports of in vitro breeding of Eucommia plants have problems such as poor repeatability, low proliferation efficiency of adventitious buds, and low rooting efficiency, which seriously limit the germplasm preservation and large-scale propagation of Eucommia improved varieties

Method used

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  • Efficient eucommia ulmoides regeneration method based on LED light quality regulation and control
  • Efficient eucommia ulmoides regeneration method based on LED light quality regulation and control
  • Efficient eucommia ulmoides regeneration method based on LED light quality regulation and control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The selection of Eucommia explant disinfection solution is as follows:

[0038] 1. Select an excellent strain of perennial eucommia ulmoides that grows well from the field test field, and cut the non-lignified branches of the current year as the source of explants.

[0039] 2. After removing the leaves, the branches are cut into 5cm-sized stem segments, and rinsed under running water for 20 minutes; after being wiped once with 75% absolute ethanol, they are rinsed with sterile water 5 times in a sterile operating table; then rinsed with 75% Disinfect with absolute ethanol for 45s, rinse with sterile water 4 times; then use 0.1% HgCl 2 solution, 10% H 2 o 2 The solution and 10% NaClO disinfectant were disinfected for 1, 3, 5, and 7 minutes respectively, and finally rinsed 6 times with sterile water. Shake constantly during disinfection. After disinfection, the necrotic parts at both ends of the stem segment are excised, and then the stem segment is cut into sections ...

Embodiment 2

[0041] The regeneration system of different genotypes of Eucommia was established, and the specific operations were as follows:

[0042]1. Select good-growing perennial triploid and diploid Eucommia Eucommia strains from the field test field, and cut un-lignified branches of the current year as the source of explants.

[0043] 2. After removing the leaves, the branches are cut into 5cm-sized stem segments, and rinsed under running water for 20 minutes; after being wiped once with 75% absolute ethanol, they are rinsed with sterile water 5 times in a sterile operating table; then rinsed with 75% Disinfect with absolute ethanol for 45s, rinse with sterile water 4 times; then use 0.1% HgCl 2 The solution was disinfected for 3 minutes, and finally rinsed 6 times with sterile water. Shake constantly during disinfection. After disinfection, the necrotic parts at both ends of the stem segment are excised, and then the stem segment is cut into sections with at least one axillary bud ...

Embodiment 3

[0046] The promotion effect of different LED light qualities on the germination of axillary buds of Eucommia stems, the specific operations are as follows:

[0047] 1. Select an excellent strain of perennial eucommia ulmoides that grows well from the field test field, and cut the non-lignified branches of the current year as the source of explants.

[0048] 2. After removing the leaves, the branches are cut into 5cm-sized stem segments, and rinsed under running water for 20 minutes; after being wiped once with 75% absolute ethanol, they are rinsed with sterile water 5 times in a sterile operating table; then rinsed with 75% Disinfect with absolute ethanol for 40s, rinse with sterile water 4 times; then use 0.1% HgCl 2 The solution was disinfected for 3 minutes, and finally rinsed 6 times with sterile water. Shake constantly during disinfection. After disinfection, the necrotic parts at both ends of the stem segment are excised, and then the stem segment is cut into sections ...

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Abstract

The invention provides an efficient eucommia ulmoides regeneration method based on LED light quality regulation and control, which comprises the following steps: taking current-year stems with axillary buds of an excellent eucommia ulmoides triploid strain as explants, performing surface disinfection, and performing germination, proliferation, elongation and rooting induction culture on the axillary buds to finally obtain complete regenerated plants of eucommia ulmoides. An appropriate LED light quality ratio with long service life and low energy consumption is introduced in eucommia ulmoides tissue culture rapid propagation, so that the energy consumption cost is reduced, the propagation coefficient of eucommia ulmoides tissue culture seedlings is improved, the elongation time of adventitious buds is shortened, the tissue culture seedlings are dark green in leaves and relatively rapid in growth, a foundation is laid for rapid large-scale propagation of eucommia ulmoides excellent strains, and meanwhile, an important technical support is provided for genetic improvement of eucommia ulmoides by utilizing a molecular biology means in the later period.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a high-efficiency regeneration method of Eucommia ulmoides based on LED light quality control. Background technique [0002] Eucommia ulmoides Oliver (Eucommia ulmoides Oliver) is a perennial woody plant of the genus Eucommia in the family Eucommia. It is a relic plant left in the geological history that has only survived in central China. It is a unique living fossil plant in China. It has the same medicine and food, and it is a national second-class protected plant. In addition, Du is a national strategic resource, precious medicinal materials, woody oil tree species, and high-quality natural rubber resources. It is also an important tree species for maintaining ecological security, increasing carbon sinks, timber reserves, and realizing green farming. Because it contains active ingredients such as chlorogenic acid and aucanthoside, it has pharmacological effects such as regulating ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02P60/14
Inventor 吴丽芳王大成侯金艳丁双双苏鹏飞王萍
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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