Online real-time detection system and method for biological sample

Abstract

The invention provides an online real-time detection system and detection method for a biological sample. The online real-time detection system for the biological sample comprises a culture container,a sampling pipe fitting, a quantitative assembly, a sampling host, an analyzer, a material supplementing assembly and a control part. The sampling pipe fitting is used for sampling a to-be-detected sample from the culture container to the quantitative assembly; the quantifying assembly comprises a quantifying structure, and the quantifying structure is used for determining the amount of the to-be-detected sample sampled from the culture container to the quantifying assembly; the sampling host comprises a main pump, and the main pump is used for driving the to-be-detected sample from the culture container to the quantitative assembly and driving the to-be-detected sample determined by the quantitative structure to the analyzer; the analyzer is used for receiving the to-be-detected sample and analyzing the to-be-detected sample to obtain an analysis result; the material supplementing assembly is connected to the feeding hole of the culture container through a pipeline; the main pump, the analyzer and the material supplementing assembly are all in signal connection with the control part, and the control part is used for obtaining the analysis result and controlling the material supplementing assembly to supplement materials to the culture container according to the analysis result.

Description

technical field [0001] The application belongs to the technical field of biological sample detection, and more specifically relates to an online real-time detection system and method for biological samples. Background technique [0002] In the prior art, when testing the culture medium in industries such as microbial culture and cell culture, it is first necessary to manually take out the sample from the culture container, then put the sample into a centrifuge for centrifugation, and then take out the supernatant. Manually transfer the supernatant to the analysis equipment for detection. After the detection results are obtained through detection, it is judged whether to feed the culture medium in the container. If feeding is required, the feeding equipment is manually controlled to feed. [0003] Specifically, microorganism culture, cell culture and other industries currently use culture tanks made of glass or stainless steel, and the size of the tank varies from several mi...

AI Technical Summary

Problems solved by technology

[0005] First, the sterilization operation before and after sampling is cumbersome and time-consuming, which cannot meet the high sampling frequency requirements;

[0006] Second, the entire detection process includes manual sampling, centrifugation, detection, and feeding. It takes a long time to consume, and the component content of the tested items in the cultivation tank is constantly changing. The content of the detected items may have changed, thus causing the feed to fail to achieve the expected effect;

[0007] Third, the amount of manual sampling cannot be precisely controlled, and for the cell culture industry with less culture medium, multiple sampling and monitoring cannot be achieved;

[0008] Fourth, the entire existing detection process requires manual participation in the operation, which consumes a lot of labor costs

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