IncRNA molecule and application thereof

A molecular and genetic technology, applied in the field of lncRNA molecules, can solve problems such as insufficient research

Active Publication Date: 2021-09-17
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still insufficient research on this

Method used

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  • IncRNA molecule and application thereof
  • IncRNA molecule and application thereof
  • IncRNA molecule and application thereof

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Experimental program
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Embodiment 1

[0030] 1 material

[0031] RNAiso Plus Total RNA Extraction Reagent (Takara 9109);

[0032] RNA reverse transcription kit (ROCHE 04897030001);

[0033] SYBgreen method qPCR kit (ROCHE 04913914001);

[0034] High-fidelity enzyme PrimeSTAR Max DNA Polymerase (Takara R045A);

[0035] RACE 5' / 3' Kit (TAKARA 634858).

[0036] 2 methods

[0037] 2.1 Identification of the full-length sequence of the new transcript of TCONS_00013523

[0038] 2.1.1 5'RACE and 3'RACE amplification primer design

[0039] The human lncRNA TCONS_00013523 sequence was downloaded from the UCSC database, and primers for nested PCR were designed for 5'RACE and 3'RACE amplification. Amplification primers were synthesized by Suzhou Jinweizhi Biotechnology Co., Ltd., and the primer sequences are shown in Table 1.

[0040] Table 1 Sequences of primer pairs for 5'RACE and 3'RACE amplification

[0041]

[0042]

[0043] 2.1.2 5’RACE and 3’RACE amplification and sequencing

[0044] see RACE 5' / 3'...

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Abstract

The invention relates to an lncRNA molecule and application thereof. A new transcript full-length sequence of lncRNA (TCONS-00013523) is identified in paclitaxel-resistant ovarian cancer cells and is named as PRALR-alpha, cell lines for knocking out and overexpressing the PRALR-alpha are further constructed, and the fact that the sensitivity of the ovarian cancer cells to paclitaxel (PTX) can be increased and reduced by knocking out and overexpressing the PRALR-alpha is found, and it proves that PRALR-alpha is closely related to the paclitaxel drug resistance of the ovarian cancer cells. Through the research, a new thought and method can be provided for the treatment of ovarian cancer paclitaxel chemotherapy, and a molecular targeting drug aiming at a PRALR-alpha specific target spot is synthesized; a candidate drug for treating the paclitaxel drug-resistant ovarian cancer can be screened by using a PRALR-alpha overexpression vector or a cell line; and meanwhile, the molecular mechanism generated by ovarian cancer drug resistance can be deeply researched.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a novel lncRNA molecule related to drug resistance of ovarian cancer and its application. Background technique [0002] Ovarian cancer is one of the common malignant tumors of female reproductive organs, and its mortality rate ranks first among all gynecological malignant tumors. At present, paclitaxel (PTX) chemotherapy is the first-line treatment for advanced ovarian cancer, but the recurrence of ovarian cancer caused by chemotherapy resistance is still an urgent clinical problem to be solved. [0003] lncRNA, that is, long non-coding RNA, is a general term for a class of RNA molecules with a length of more than 200 nt and basically no protein-coding function. They are widely involved in the physiological and pathological processes of the body, including the occurrence and development of tumors and chemotherapy resistance. [0004] An lncRNA (TCONS_00013523) sequence was i...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/85C12N15/90C12N5/10A61K45/00A61K48/00A61P35/00A61P15/08C12Q1/6886
CPCC12N15/113C12N15/85C12N15/907C12N5/0693A61K45/00A61K48/00A61P35/00A61P15/08C12Q1/6886C12N2310/11C12N2800/107C12N2510/00C12Q2600/158C12Q2600/178C12Q2600/136Y02A50/30
Inventor 许国雄管文彩
Owner JINSHAN HOSPITAL FUDAN UNIV
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