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Method for guiding nanoparticle superlattice by using DNA origami and DNA tiles

A nanoparticle and superlattice technology, applied in nanotechnology, nanomedicine, nanotechnology, etc., can solve the problems of limiting the adjustment of DNA origami spacing, soft crystals are easily affected by the environment, etc., to improve rigidity and compression resistance, The effect of improving overall rigidity

Active Publication Date: 2021-09-21
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The DNA origami units are connected by hybridization and complementarity between the single-stranded DNA protruding from the vertices. In addition to the complementary sequences, the single strands also include poly T sequences that adjust the distance between the origami units. The distance between adjacent DNA origami units Single-strand-dominated spacer regions greatly limit regulation of DNA origami spacing and lead to soft crystals susceptible to environmental influences

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  • Method for guiding nanoparticle superlattice by using DNA origami and DNA tiles
  • Method for guiding nanoparticle superlattice by using DNA origami and DNA tiles
  • Method for guiding nanoparticle superlattice by using DNA origami and DNA tiles

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Embodiment 1

[0024] A method of using DNA origami and DNA tiles to guide the nanoparticle superlattice of the present invention comprises the following steps:

[0025] (1) Synthesis of DNA origami octahedra: by mixing 10 nM M13mp18 scaffold DNA and 144 single-stranded DNA in a buffer containing 1 mM EDTA, 12.5 mM magnesium acetate, and cooling the mixed solution from 90 °C to room temperature for more than 20 hours Slow annealing to fold DNA origami octahedral framework; the molar ratio of M13mp18 scaffold DNA to single-stranded DNA is 1:10.

[0026] (2) Synthesis of DNA tile structure: by mixing complementary ssDNA in an equimolar ratio in 0.3 M PBS buffer, cooling the mixture from 95°C to room temperature within two days; DNA tile structure was synthesized; the PBS buffer Contains 0.3 M NaCl, 10 mM phosphate. The DNA tile structure is double-stranded DNA.

[0027] (3) Synthesis of 3D crystalline nanoparticle superlattices: DNA origami octahedrons prepared as described above were mixed ...

Embodiment 2

[0029] The difference between embodiment 2 and embodiment 1 is:

[0030] A method of using DNA origami and DNA tiles to guide the nanoparticle superlattice of the present invention comprises the following steps:

[0031] (1) Synthesis of DNA origami octahedra: by mixing 10 nM M13mp18 scaffold DNA and 144 single-stranded DNA in a buffer containing 1 mM EDTA, 12.5 mM magnesium acetate, and cooling the mixed solution from 90 °C to room temperature for more than 20 hours Slow annealing to fold the DNA origami octahedral framework; the molar ratio of M13mp18 scaffold DNA to single-stranded DNA is 1:20.

Embodiment 3

[0033] The difference between embodiment 3 and embodiment 1 is:

[0034] A method of using DNA origami and DNA tiles to guide the nanoparticle superlattice of the present invention comprises the following steps:

[0035] (1) Synthesis of DNA origami octahedra: by mixing 10 nM M13mp18 scaffold DNA and 144 single-stranded DNA in a buffer containing 1 mM EDTA, 12.5 mM magnesium acetate, and cooling the mixed solution from 90 °C to room temperature for more than 20 hours Slow annealing to fold the DNA origami octahedral framework; the molar ratio of M13mp18 scaffold DNA to single-stranded DNA is 1:15.

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Abstract

The invention discloses a method for guiding a nanoparticle superlattice by using DNA origami and DNA tiles. The method comprises the following steps of (1) synthesizing a DNA origami octahedron, (2) synthesizing a DNA tile structure, and (3) synthesizing the three-dimensional crystal nanoparticle superlattice. The DNA origami units are used for constructing the three-dimensional crystal, the distance between the DNA origami units is flexibly adjusted by replacing single chains between the origami units with different DNA tile structures, and the rigidity and pressure resistance of the three-dimensional soft crystal are improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for guiding nanoparticle superlattices by using DNA origami and DNA tiles. Background technique [0002] It has been reported in the literature that DNA origami frameworks are used to directly form three-dimensional crystals. For example, in 2020, Oleg Gang's group reported in Nature Materials that a DNA origami octahedron was used to guide the formation of three-dimensional crystals. They regard the origami octahedron as a basic unit, and the single-stranded DNA protruding from each vertex hybridizes with each other to construct a strict three-dimensional array, thereby realizing the formation of a three-dimensional crystal of the origami framework. [0003] (Literature: Ordered three-dimensional nanomaterials using DNA-prescribed and valence-controlled material voxels | pages789–796(2020) | NatureMaterials) [0004] The DNA origami units are connected by hybridization a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C30B29/58C30B29/68C30B7/14C30B7/08B82Y5/00B82Y40/00
CPCC30B29/58C30B29/68C30B7/14C30B7/08B82Y5/00B82Y40/00
Inventor 田野陈志马宁宁
Owner NANJING UNIV
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