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Whole-cell component conveying system and application thereof

A delivery system, the technology of whole cells, applied in the application of preventive and therapeutic vaccines, the field of delivery system of whole cell components

Active Publication Date: 2021-09-28
SUZHOU ERSHENG BIOPHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no way to use whole cell components of cancer cells as a vaccine for the prevention and treatment of cancer

Method used

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  • Whole-cell component conveying system and application thereof
  • Whole-cell component conveying system and application thereof
  • Whole-cell component conveying system and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1. Whole cell components of melanoma cancer cells are loaded inside and on the surface of nanoparticles for cancer treatment

[0090] This example uses mouse melanoma as a cancer model to illustrate how to prepare a nano-vaccine loaded with whole cell components, and apply the vaccine to treat melanoma.

[0091] PD-1 antibody has been approved for the treatment of many cancers because of its excellent clinical efficacy. For this reason, in this example, in addition to using only the nano-vaccine to treat melanoma, the applicant also tested the combination of nano-vaccine and PD-1 antibody to treat melanoma. In actual application, the specific administration time, administration frequency, and administration regimen can be adjusted according to the situation.

[0092] In this example, B16F10 mouse melanoma cells were used as cancer cell models. B16F10 was first lysed to prepare water-soluble and water-insoluble fractions of B16F10 cells. Then, the organic poly...

Embodiment 2

[0108] Example 2. Water-soluble cell components of melanoma cancer cells loaded inside and on the surface of nanoparticles for cancer treatment

[0109] This example uses mouse melanoma as a cancer model to illustrate how to prepare a nano-vaccine loaded with only the water-soluble part of the cell components, and use the vaccine to treat melanoma.

[0110] PD-1 antibody has been approved for the treatment of many cancers because of its excellent clinical efficacy. For this reason, in this example, the applicant applied the nano-vaccine in combination with PD-1 antibody to treat melanoma. In practical application, the nano-vaccine prepared by only using the original water-insoluble part in the cell component can also be used for treatment. In addition, the specific administration time, administration frequency, and administration regimen can be adjusted according to the situation.

[0111] In this example, B16F10 mouse melanoma cells were used as cancer cell models. B16F10 ...

Embodiment 3

[0125] Example 3, Melanoma Tumor Tissue Lysis Components Loaded Inside and on the Surface of Nanoparticles for Cancer Treatment

[0126] This example uses mouse melanoma to illustrate how to prepare a nano-vaccine loaded with melanoma tumor tissue lysate components, and use the vaccine to treat melanoma.

[0127] In this example, the applicant tested the effect of the combination of the nano-vaccine and PD-1 antibody in the treatment of melanoma. In practical applications, only the nano-vaccine can be used to treat cancer.

[0128] In this example, the lysed components of mouse melanoma tumor tissue were loaded inside and on the surface of nanoparticles to prepare nanovaccine. First, the mouse melanoma tumor mass was obtained and lysed to prepare the water-soluble fraction of the tumor mass tissue and the original water-insoluble fraction dissolved in 8M urea. Then, using PLGA as nanoparticle framework material and poly(I:C) as immune adjuvant, the nanovaccine loaded with wat...

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Abstract

The application belongs to the field of immunotherapy and discloses a conveying system for conveying water-soluble components and non-water-soluble components of whole-cell components by using nano-scale or micron-scale particles and application of the conveying system in preparation of vaccines for preventing and treating cancers. The whole-cell component conveying system is composed of the nano-scale or micron-scale particles and the whole-cell components loaded by the particles, wherein the whole-cell components are water-soluble components and non-water-soluble components of whole cells in cells or tissues. The water-soluble components and the non-water-soluble components are loaded in the nano particles or the micron particles, so that variant proteins or polypeptides generated by the cancers in the cell components are loaded in the nano particles or the micron particles. Substances with immunogenicity, which are generated due to disease mutation, in the whole-cell components can be used for preventing and treating the cancers. The whole-cell component conveying system can be used for preparing the vaccines for preventing and / or treating the cancers.

Description

technical field [0001] The invention belongs to the field of immunotherapy, and in particular relates to a delivery system of whole cell components and its application, in particular to a delivery system of whole cell components and its application in preparing preventive and therapeutic vaccines for cancer. Background technique [0002] Immunity is a physiological function of the human body. The human body relies on this function to identify "self" and "non-self" components, thereby destroying and repelling antigenic substances (such as viruses and bacteria) entering the human body, or damaged cells produced by the human body itself. and tumor cells to maintain human health. Immunotechnology has developed extremely rapidly in recent years, especially in the field of cancer immunotherapy. With the continuous improvement of the understanding of cancer, people have found that the human immune system and various immune cells play a key role in the process of inhibiting the occ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K39/39A61K39/395A61K9/14A61K9/51A61K9/16A61K9/50A61K47/34A61P35/00
CPCA61K39/0011A61K39/39A61K39/39558A61K9/146A61K9/5153A61K9/1647A61K9/5031A61P35/00A61K2039/5154A61K39/00A61K39/00119A61K2039/5152A61K2300/00A61K2039/55555A61K2039/55561A61K2039/876A61K2039/812A61K2039/6006A61K2039/523C07K16/2818A61K2039/505A61K39/3955A61K2039/545A61K2039/54Y02A50/30A61K9/513A61K9/1629A61K39/385A61K9/1658A61K9/1611A61K47/6937A61P17/00A61K2039/55594
Inventor 刘密
Owner SUZHOU ERSHENG BIOPHARMACEUTICAL CO LTD
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