Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting gene mutation related to anti-oxidative stress pathway of lung adenocarcinoma

An anti-oxidative stress and pathway-related technology is applied in the fields of genomics, biochemical equipment and methods, and microbial measurement/testing, which can solve the problems of expensive detection and achieve good application prospects, high accuracy, sensitivity and good specific effect

Active Publication Date: 2021-09-28
ZHONGSHAN HOSPITAL FUDAN UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, the detection of gene mutations related to the antioxidant stress pathway in lung adenocarcinoma is not a routine clinical item, and the detection alone is expensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting gene mutation related to anti-oxidative stress pathway of lung adenocarcinoma
  • Kit for detecting gene mutation related to anti-oxidative stress pathway of lung adenocarcinoma
  • Kit for detecting gene mutation related to anti-oxidative stress pathway of lung adenocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1 Screening and Effect Verification of Gene Sets

[0102] (1) Construction of scoring model for gene mutations related to oxidative stress pathway in lung adenocarcinoma

[0103] 1. Method

[0104] Firstly, the data of 493 lung adenocarcinoma samples were obtained from the TCGA database, and the samples were divided into mutation group (n=111) and wild group (n=382) according to the presence or absence of cell cycle progression-related gene (KEAP1, NFE2L2, CUL3) mutations, By performing LASSO regression analysis on the expression data of all genes in the full RNA expression profile of 493 samples in [log2(FPKM+1) form], the attached figure 2 Plot of binomial deviance as a function of log(λ) in LASSO regression ( figure 2 ), under different values ​​of λ, the prediction effect of the model of the obtained characteristic variables is also different, according to image 3 It can be seen that when choosing figure 2 When the model with the smallest mean square...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kit for detecting gene mutation related to an anti-oxidative stress pathway of lung adenocarcinoma. The kit contains detection reagents for detecting the expression quantity of the following genes: RP11-539L10.2, AKR1C2, RP11-572H4.1, TRIM16L, RARA, SESN2, RP5-827L5.2, CTD-2139B15.5, Metazoa_SRP, snoU13, RP11-545H22.1, KRT8P30, TALDO1, TRAPPC13P1, GS1-388B5.2, RP11-267L5.1, TRAV11, RP11-699A7.1 and AL132671.1. Compared with the prior art, the kit has the advantages that the genes related to the anti-oxidative stress pathway are screened through RNA sequencing, LASSO and binary classification Logistic regression, the score Score is constructed, the cut-off value of the corresponding score in the lung adenocarcinoma is obtained through ROC curve analysis, and the kit can be used for predicting mutation of the genes (KEAP1, NFE2L2 and CUL3) related to the anti-oxidative stress pathway in the lung adenocarcinoma. The specific gene mutation of the lung adenocarcinoma is predicted by utilizing the expression quantity of the gene composition, the prediction method has the advantages of high accuracy and good specificity through verification of a TCGA (The Cancer Genome Atlas) database, an experiment and a multi-omics database, and the kit has a very good application prospect.

Description

technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a kit for detecting gene mutations related to the anti-oxidative stress pathway of lung adenocarcinoma. Background technique [0002] As the most common malignant tumor, lung cancer has the highest mortality rate among all tumors in the world. Lung adenocarcinoma is the most common subtype of lung cancer, accounting for more than 40% of lung cancer cases. The emergence of various targeted drugs against lung adenocarcinoma has significantly improved the prognosis of patients with lung adenocarcinoma, and the treatment of lung adenocarcinoma has entered the molecular era. How to accurately assess the molecular characteristics of lung adenocarcinoma is of great significance for the precise treatment of patients and the reduction of social burden. [0003] The anti-oxidative stress pathway plays a huge role in the occurrence and development of lung cancer. Although unde...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886C12N15/11G16B20/50
CPCC12Q1/6886G16B20/50C12Q2600/156
Inventor 金星郑元生陈振淙卞赟艺毕国澍单光耀黄宜炜赵梦男詹成王群
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com