Design kit for targeted detection of small round cell tumor fusion gene panel of soft tissue tumor

Abstract

The invention belongs to the technical field of gene sequencing, and discloses a next-generation sequencing kit for targeted detection of formalin-immobilized paraffin embedded (FFPE) soft tissue small round cell tumor fusion genes. The kit comprises a DNA extraction reagent for DNA extraction; an RNA extraction and transcription reagent is used for cDNA acquisition; a multiplex PCR primer and a multiplex PCR amplification reagent of the 30 gene are used for specific amplification of the 30 gene; dNA purification magnetic beads are used for purifying the product; a library building reagent is used for building a DNA / cDNA library for next-generation sequencing. The 30 gene multiple PCR primers are synthesized, then primers are synthesized in related biological companies, multiple PCR is completed under the condition that mismatching of DNA melting temperature is reduced to the maximum extent, and detection of amplification of target fusion genes is completed; the reagents used in the invention can be prepared through purchased commercial reagents or can be directly purchased. The invention can provide direct genetic basis for diagnosis and differential diagnosis of various tumors in soft tissue small round cell tumors.

Description

technical field [0001] The invention belongs to the technical field of gene sequencing, in particular to a panel design kit for targeted detection of soft tissue tumor small round cell tumor fusion genes. Background technique [0002] At present, in clinical practice, soft tissue small round cell tumors, such as extraskeletal Ewing sarcoma, alveolar rhabdomyosarcoma, and differentially differentiated synovial sarcoma, have certain morphological similarities, making diagnosis relatively difficult. In recent years, with the advancement of molecular detection methods, some characteristic fusion genes have been discovered in soft tissue small round cell tumors, providing a strong molecular basis for the diagnosis of soft tissue small round cell tumors. [0003] However, traditional exome sequencing and whole genome sequencing are expensive, the sequencing depth is not enough, the pertinence is poor, the sample requirements are high, and the ability to detect specific fusion gene...

AI Technical Summary

Problems solved by technology

[0004] Through the above analysis, the problems and defects of the existing technology are: traditional exome sequencing and whole genome sequencing are expensive, the sequencing depth is not enough, the pertinence is poor, the requirements for samples are high, and the ability to detect specific fusion genes is limited

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