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Cell-free protein synthesis platforms derived from clostridia

A protein synthesis, Clostridium technology, applied in protein nucleotide library, microbial-based methods, bacteria, etc., can solve low-throughput, labor-intensive and time-consuming problems

Pending Publication Date: 2021-10-08
NORTHWEST UNIV(CN) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Clostridia have been used industrially for over 100 years, current strain engineering techniques remain low-throughput, labor-intensive, and time-consuming challenges

Method used

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  • Cell-free protein synthesis platforms derived from clostridia
  • Cell-free protein synthesis platforms derived from clostridia
  • Cell-free protein synthesis platforms derived from clostridia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0120] Embodiment 1. A cell-free protein synthesis platform for in vitro transcription of mRNA and / or translation of polypeptides comprising as a component a cell extract prepared from a cell culture of a Clostridium species.

Embodiment approach 2

[0121] Embodiment 2. The platform of embodiment 1, wherein the Clostridium species is selected from the group consisting of Clostridium ethanologenes, Clostridium ljungdahlii, Clostridium butyricum, Clostridium bejejejerinckii, Clostridium pasteuriani, Clostridium saccharobutyricum (Clostridium saccharoperbutylacetonicum), Clostridium aceticum, Clostridium tetanomorphum, Clostridium phytofermentans, Clostridium arbusti, Clostridium akagii, Clostridium diolis, Clostridium acetireducens, Clostridium coskatii, Clostridium ragsdaleium, Clostridium drakeum scakeiformico, Clostridium krustridium, Clostridium tyrobutyricum, Clostridium grantii, Clostridium homopropionicum, Clostridium tepidiprofundi, Clostridium collagenovorans, Clostridium tunisiense, Clostridium argentinense, Clostridium ihumii, Clostridium cadaveris, Clostridium amylolyticum, Clostridium sartagoforme, Pasteurella , Clostridium paraputrificum, Clostridium fallax, Clostridium cavendishii, Clostridium cylindrosporum, ...

Embodiment approach 3

[0122] Embodiment 3. The platform of embodiment 1 or 2, wherein the Clostridium species is Clostridium ethanogenum.

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Abstract

Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and / or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from a species of Clostridia such as cellular extracts from Clostridium autoethanogenum.

Description

[0001] Statement Regarding Federally Sponsored Research or Development [0002] This invention was made with government support under SC0018249 awarded by the US Department of Energy. The government has certain rights in this invention. [0003] Cross References to Related Applications [0004] This application claims priority under 35 U.S.C § 119(e) to U.S. Provisional Application No. 62 / 810,014, filed February 25, 2019, the contents of which are incorporated herein by reference in their entirety. Background technique [0005] The present invention generally relates to compositions, methods and kits for performing cell-free RNA transcription and / or cell-free protein synthesis (CFPS). More specifically, the present invention relates to compositions, methods and kits for performing cell-free RNA transcription and / or performing cell-free protein synthesis (CFPS) comprising or utilizing natural Components produced from existing or recombinant species. [0006] Clostridium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12N1/21C40B40/08C12R1/145
CPCC12N1/20C12N9/1247C12Y207/07006C12N15/1051C12P21/02C12N15/52C12P21/00C12N15/1072
Inventor M·C·朱厄特A·克鲁格-格里克A·P·穆勒M·科普克
Owner NORTHWEST UNIV(CN)
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