Production method and equipment for preparing ethanol through CO fermentation

A production method, ethanol technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem that the hydrolyzate is not effectively used, achieve the goals of shortening the production cycle, simple method, and improving the conversion rate of ethanol Effect

Active Publication Date: 2015-07-01
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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Problems solved by technology

On the other hand, when lignocellulosic biomass is treated with high-temperature liquid water, the obtained hydrolyzate mainly contains xylose and xylo-oligosaccharides, and the content of fermentation inhibitors such as furfural is low, but the hydrolyzate has not been effectively utilized so far.

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  • Production method and equipment for preparing ethanol through CO fermentation

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Embodiment

[0034] see figure 1 As shown, lignocellulosic raw materials such as bagasse, sweet sorghum bagasse, etc. are crushed to 40-80 mesh, mixed with water at a mass ratio of 1:20, and placed in hydrolysis reactor 2 for hydrolysis. Reaction conditions: 180°C, 4MPa, 20min (pass N 2maintain pressure). After the reaction, the hydrolyzate is cooled to about 40° C. through the cooler 4 and enters the culture medium storage tank 6 . After the hydrolysis residue is taken out from outlet 3, it can be further enzymatically fermented. Add other medium components to the medium storage tank 6 from the inlet 7 and mix with the hydrolyzate. The prepared culture medium enters the sterilizer 8 and is sterilized at 121° C. for 15 minutes.

[0035] Both the reactor A10 and the reactor B16 are cylinders with the same volume, but the diameter of the reactor B16 is half of the diameter of the reactor A10. Reactor A10 and Reactor B16 were pre-steam sterilized. The sterilized culture medium is passed...

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Abstract

The invention discloses production method and equipment for preparing ethanol through CO fermentation. The fermentation is performed by a two-step process, and the growth and fermentation are separately performed by adopting different reactors and reaction conditions respectively. The hydrolysate obtained after high-temperature liquid water treatment on lignocellulose biomass is used for providing a carbon source to the strain growth, and CO serves as a unique carbon source in ethanol fermentation; a stirring pot-type reactor is adopted in the growth stage, and a packed bed type reactor is adopted in the fermentation stage; the culture liquid from the stirring pot type reactor is directly input into the packed bed type reactor without needing cell separation concentration and supplementation of a new culture medium. The fermentation strain is clostridium autoethanogenum. In the production method and equipment disclosed by the invention, by adopting an improved culture medium, the cost of the culture medium is reduced, the comprehensive utilization of the lignocellulose biomass hydrolysate is realized, and the process is simplified. Moreover, since CO is not applied to thallus growth but the fermentation stage only, the whole production period can be shortened, and the ethanol conversion rate of CO is greatly improved.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a production method and equipment for producing ethanol by CO fermentation. technical background [0002] With the rapid development of industry, the discharge of industrial waste gas is increasing day by day, and the toxic and harmful gases contained in these waste gases make the environmental pollution more and more serious. For example, CO, which is a toxic gas that widely exists in industrial production, such as blast furnace gas, converter gas, coke oven gas, calcium carbide furnace gas, yellow phosphorus furnace gas, carbon black tail gas, etc., all contain CO, and the CO content in these waste gases It can be from a few percent to about 90% (volume ratio). It is conservatively estimated that the annual CO emissions from my country's iron and steel industry alone can reach at least 50 million tons. How to control or effectively utilize these industrial waste gases is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12M1/04C12M1/02C12R1/145
CPCY02E50/10
Inventor 袁振宏徐惠娟许敬亮梁翠谊庄新姝孙永明余强郭颖
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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