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Aquatic feed containing clostridium autoethanogenum protein and production process of aquatic feed

A technology of Clostridium ethanolicum and aquatic feed, which is applied in the field of freshwater fish breeding, can solve the problems of poor product quality, amino acid digestibility, amino acid imbalance, etc., and achieves the effects of good economic benefits, completeness, good balance, and high protein content.

Pending Publication Date: 2021-04-09
TONGWEI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant protein is the cheapest and most abundant alternative protein source, but plant protein has disadvantages such as poor palatability, amino acid imbalance and many anti-nutritional factors
Therefore, it is possible to look for bacterial proteins with low anti-nutritional factor content, such as single-cell protein, but the quality of bacterial protein products is largely affected by the content of non-protein nitrogen, and some products are of poor quality, with low true protein content, amino acid Imbalances and Amino Acid Digestibility Issues
[0005] At present, there are few studies on single-cell protein source fishmeal, and there is a lot of room for development

Method used

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  • Aquatic feed containing clostridium autoethanogenum protein and production process of aquatic feed
  • Aquatic feed containing clostridium autoethanogenum protein and production process of aquatic feed
  • Aquatic feed containing clostridium autoethanogenum protein and production process of aquatic feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The production of embodiment 1 ethanol clostridium protein

[0028] A kind of clostridium ethanol protein production process, comprises the following steps:

[0029] (1) Cultivate Clostridium autoethanogenum in the culture medium, and select mature Clostridium autoethanogenum as the fermentation strain; the Clostridium autoethanogenum has a preservation number of DSM10061 and a preservation address of the German Culture Collection, DSMZ.

[0030] (2) Pour the ethanol fermentation raw material into the fermenter, the ethanol fermentation raw material is in a liquid state, add the fermentation strain into the fermenter and mix with the ethanol fermentation raw material.

[0031] (3) Pass CO gas into the fermenter, CO gas discharges the air in the fermenter and fills the space in the fermenter, and then seals the exhaust port of the fermenter; a gas detection device is provided at the vent port of the fermenter If the gas discharged from the outlet is CO, seal the outlet ...

Embodiment 2

[0035] Preparation and fish feeding of embodiment 2 fish feed

[0036] The preparation method of the fish feed is as follows: uniformly mix the ethanol clostridial protein, other raw materials and water to obtain a mixed material, make the mixed material into granules, and dry the fish feed to obtain the fish feed.

[0037] A total of 540 carps with an initial body weight of (25.67±0.03) g were selected and randomly divided into 6 groups with 3 replicates in each group and 30 fish in each replicate. The control group (J0 group) was fed with basic feed, and the test group was fed with 3% (J3 group), 6% (J6 group), 9% (J9 group), 12% (J12 group), and 15% (J15 group) respectively. Clostridium ethanol protein was used to replace the fish meal in the basal feed in equal amounts, and the breeding cycle was 90 days. The specific formula is shown in Table 1, the growth of fish is shown in Table 2, and the biochemical indicators of fish plasma are shown in Table 3.

[0038] Table 1 F...

Embodiment 3

[0050] Embodiment 3 enlarges and replaces experiment

[0051] In order to further verify the effect of the replacement of Clostridium ethanol protein and fish meal on the growth performance and serum biochemical indicators of fish, more experiments were carried out.

[0052] The preparation method of fish feed is identical with embodiment 2

[0053] A total of 900 carps with an initial body weight of (25.67±0.03) g were selected and randomly divided into 10 groups, with 3 replicates in each group and 30 fish in each replicate. Respectively with 1% (T1 group), 2% (T2 group), 3% (T3 group), 4% (T4 group), 5% (T5 group), 6% (T6 group) of Clostridium ethanol in equal amounts Replace the fish meal in the basal feed, and the breeding cycle is 90 days; at the same time, in order to study whether the Clostridium ethanol protein replaces the fish meal, whether it has synergistic / antagonistic effect with other raw materials, increase 4 groups to use 3% (T3-1 group), 3% ( T3-2 group), ...

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Abstract

The invention discloses an aquatic feed containing clostridium autoethanogenum protein and a production process of the aquatic feed, and belongs to the technical field of freshwater fishes. The special feed contains fish meal and clostridium autoethanogenum protein. The clostridium autoethanogenum protein partially replaces fish meal and is added into the aquatic feed; wherein the replacement amount of the clostridium autoethanogenum protein is less than or equal to 6% of the total mass of the aquatic feed. The production process comprises the following steps: uniformly mixing the raw materials, preparing into granules, and drying to obtain the aquatic feed containing the clostridium autoethanogenum protein. The fish feed is developed on the basis that the clostridium autoethanogenum strain does not generate spores and toxic genes, belongs to a risk grade group I, is isolated from the environment, is not subjected to gene modification, and further has the advantages of high protein content, complete amino acid varieties, good balance, high digestibility and the like. Fish meal can be partially replaced, and dependence on fish meal is reduced; good economic benefits are realized.

Description

technical field [0001] The invention relates to the technical field of freshwater fish feeding, in particular to an aquatic feed containing Clostridium ethanol protein and a production process thereof. Background technique [0002] With the development of the world's feed industry and aquaculture industry, the demand for fishmeal continues to increase. In recent years, due to the impact of overfishing, environmental pollution and El Nino phenomenon and other adverse climates, wild fishmeal resources have been decreasing, resulting in a decline in world fishmeal production. Therefore, there is an urgent need to seek new protein sources to replace fishmeal. [0003] Fishmeal substitutes mainly include animal protein sources, vegetable protein sources and single-cell protein sources: [0004] There are biosafety issues with animal protein, such as salmonella and mad cow disease. Plant protein is the cheapest and most abundant alternative protein source, but plant protein has...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/18A23K10/22A23K10/30A23K10/37A23K20/105A23K20/142A23K20/147A23K20/158A23K20/20A23K20/22A23K20/26
CPCA23K50/80A23K10/22A23K20/147A23K10/18A23K10/30A23K10/37A23K20/142A23K20/158A23K20/26A23K20/22A23K20/105A23K20/30Y02P60/87Y02A40/818
Inventor 米海峰张璐王武刚尹恒王祎李宝圣
Owner TONGWEI
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