Method for rapidly extracting sulforaphane from broccoli seeds
A technology for broccoli seeds and sulforaphane, which is applied in the directions of organic chemistry, fermentation, etc., can solve the problems of complex extraction method of sulforaphane, poor extract activity, excessive use of organic reagents, etc. Simple production equipment and consumables with good properties
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Embodiment 1
[0023] Crush and sieve: Take a certain amount of broccoli seeds, grind them with a grinder, stop for 10 seconds after every 15 seconds of crushing, a total of 3 rounds, pass through a 25-mesh sieve after crushing, and then get the broccoli seed powder raw material;
[0024] Extraction: Weigh 50g broccoli seed sieve powder, add 15 times the volume of 20% ethanol solution, 750mL each time, extract in water bath at 40°C for 2 hours, extract twice in total, filter after extraction, collect and combine the filtrate;
[0025] Concentration: Concentrate the filtrate on a rotary evaporator to remove ethanol, the temperature of the water bath is 52°C, the vacuum degree is 0.1Mpa, and concentrate until the solid content is about 20%;
[0026] Drying: The concentrated juice was freeze-dried to obtain 10.55 g of broccoli seed extract.
Embodiment 2
[0028] Crush and sieve: Take a certain amount of broccoli seeds, grind them with a grinder, stop for 10 seconds after every 15 seconds of crushing, a total of 3 rounds, pass through a 25-mesh sieve in the Pharmacopoeia after crushing, and obtain the broccoli seed powder raw material;
[0029] Extraction: Weigh 60g broccoli seed sieve powder, add 17.5 times the volume of 15% ethanol solution, 1050mL each time, extract in a water bath at 30°C for 2.5h, extract twice in total, filter after extraction, collect and combine the filtrates, Determine the content of sulforaphane and calculate the extraction rate of sulforaphane in broccoli seeds to be 1.60%;
[0030] Concentration: Concentrate the filtrate on a rotary evaporator to remove ethanol, the temperature of the water bath is 56°C, the vacuum degree is 0.1Mpa, and concentrate until the solid content is about 16%;
[0031] Drying: The concentrated juice was freeze-dried to obtain 13.12 g of broccoli seed extract.
Embodiment 3
[0033] Crush and sieve: Take a certain amount of broccoli seeds, grind them with a grinder, stop for 10 seconds after every 15 seconds of crushing, a total of 3 rounds, pass through a 25-mesh sieve in the Pharmacopoeia after crushing, and obtain the broccoli seed powder raw material;
[0034] Extraction: Weigh 50g of broccoli seed powder, add 20 times the volume of 25% ethanol solution, 1000mL each time, extract in a water bath at 35°C for 1 hour, extract twice in total, filter after extraction, collect and combine the filtrates, and measure Calculation of sulforaphane content The extraction rate of sulforaphane in broccoli seeds was 1.64%;
[0035] Concentration: Concentrate the filtrate on a rotary evaporator to remove ethanol, the temperature of the water bath is 55°C, the vacuum degree is 0.1Mpa, and concentrate until the solid content is about 22%;
[0036] Drying: The concentrated juice was freeze-dried to obtain 11.02 g of broccoli seed extract.
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