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Simple and efficient laboratory fairy shrimp egg hatching method

A laboratory and shrimp egg technology, applied in climate change adaptation, fish farming, application, etc., can solve the problems of unification and inconvenient feeding

Pending Publication Date: 2021-10-22
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the hatching of brine shrimp in the laboratory has not yet formed a unified and standardized operation process, which has caused some inconvenience to the use of brine shrimp for toxicology research and feeding of other organisms

Method used

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  • Simple and efficient laboratory fairy shrimp egg hatching method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The brine shrimps hatched in this example are prepared as test organisms for subsequent acute toxicity experiments, and the specific steps are as follows.

[0017] 1. Incubation Preparation

[0018] A 1500ml beaker is selected as the incubation container, and after cleaning with tap water, the container is cleaned with distilled water.

[0019] 2. Incubation solution configuration

[0020] Add 35g of sea crystal per liter of distilled water, configure artificial seawater with a salinity of 35‰, and add 1000ml to each hatching container.

[0021] 3. Egg drop and hatching

[0022] Put 0.05g of brine shrimp eggs into each hatching container filled with hatching fluid and soak for 30 minutes, then inflate the water for 10 minutes. After aeration is completed, place the incubation container at a room temperature of about 25°C and keep it under continuous light. Use a clean disposable dropper to draw the hatching solution in the hatching container every 12 hours, and wash...

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Abstract

The invention discloses a simple and efficient laboratory fairy shrimp egg hatching method. The simple and efficient laboratory fairy shrimp egg hatching method mainly comprises the following three steps that a hatching container is disinfected and cleaned; hatching liquid is prepared; and egg putting and hatching are carried out. According to the simple and efficient laboratory fairy shrimp egg hatching method, the finiteness of conditions and spaces of hatching places is fully considered, the method for hatching fairy shrimp eggs is high in universality, easy and convenient to operate and free of additional complex conditions, and the fairy shrimp larvae can be efficiently obtained.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a simple and efficient method for hatching brine shrimp eggs in a laboratory. Background technique [0002] Artemia shrimp, also known as Artemia shrimp, brine shrimp, Artemia, its development process includes: eggs, nauplii, post-nauplii, imago and adult 5 stages. Eggs hatch into nauplii in about 18-30 hours under suitable temperature and salinity. Therefore, brine shrimp has the characteristics of short development period, fast reproduction speed, strong tolerance to adverse environment, and high economic value. At present, brine shrimp can be used as a biological bait for fish, shrimp, and crabs in aquaculture, and it is also an emerging model organism for evaluating the environmental safety of pollutants. [0003] As an important feed in aquaculture, brine shrimp eggs are easy to collect, can be frozen and transported, and it only takes about one day to hatc...

Claims

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Application Information

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IPC IPC(8): A01K61/59A01K61/17
CPCA01K61/59A01K61/17Y02A40/81
Inventor 刘宪华李贇雪康宁
Owner TIANJIN UNIV
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