Method for determining activity of DNA methyltransferase and application

Abstract

The invention belongs to the technical field of enzyme activity detection, and discloses a method for determining the activity of DNA methyltransferase and application, and the method for determining the activity of DNA methyltransferase comprises the following steps: preparing RGO-CdS: Mn NPs; preparing a carboxylated CdTe QDs and CdTe@DNA (Deoxyribose Nucleic Acid) net structure; performing gold electrode pretreatment; preparing the PEC biosensor; and performing PEC signal response detection. According to the invention, the PEC biosensor of a dual signal amplification system based on a novel nanocomposite RGO-CdS: MnNPs and CdTe@DNA network structure is prepared to detect the activity of M.SssI MTase; and the prepared biosensor realizes ultra-sensitive detection of M.SssI MTase, the detection range is 0.01-80U / mL, and the method is simple to operate and does not need complex and expensive instruments or tedious signal markers.

Description

technical field [0001] The invention belongs to the technical field of enzyme activity detection, in particular to a method and application for measuring DNA methyltransferase activity. Background technique [0002] Currently, DNA methyltransferases catalyze DNA methylation and play key roles in regulating gene expression and development. Abnormal DNA methyltransferase activity leads to abnormal DNA methylation, which is closely related to many types of diseases such as cancer. Clearly, DNA methyltransferase activity is considered a potential cancer biomarker and drug target in cancer therapy. Therefore, the development of a sensitive and selective method for the analysis of DNA methyltransferase activity and the screening of its inhibitors (antimethylation drugs) is crucial for early cancer diagnosis and treatment. [0003] Currently, traditional methods for detecting DNA methyltransferase activity include gel electrophoresis, high performance liquid chromatography, and r...

AI Technical Summary

Problems solved by technology

[0004] Through the above analysis, the existing problems and defects of the prior art are: the traditional method for detecting DNA methyltransferase activity is time-consuming, laborious, cumbersome, low-sensitivity, and requires complex and expensive instruments or cumbersome signal markers or Disadvantages such as the need for radioactive reagents

[0005] The difficulty in solving the above problems and defects is: a cumbersome pretreatment process and a complicated labeling process are required, and these steps result in prolongation of detection time and low detection sensitivity

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