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Method forminingindustrial cannabis sativa hormone regulation response genes based on metabonomics technology

A technology of industrial hemp and metabolomics, applied in the direction of scientific instruments, measuring devices, instruments, etc., can solve problems that have not been reported

Pending Publication Date: 2021-10-26
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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Problems solved by technology

The application of metabolomics technology to analyze the regulatory expression mechanism of metabolites under the regulation of hormones has been reported. Studies have also shown that hormones have special effects in regulating the accumulation and metabolism of active ingredients. The mechanism of industrial hemp metabolism under hormone regulation has not been reported yet

Method used

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  • Method forminingindustrial cannabis sativa hormone regulation response genes based on metabonomics technology
  • Method forminingindustrial cannabis sativa hormone regulation response genes based on metabonomics technology
  • Method forminingindustrial cannabis sativa hormone regulation response genes based on metabonomics technology

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Embodiment 1

[0036] A method for mining industrial hemp hormone-regulated response genes based on metabolomics technology, the method specifically includes:

[0037] Step 1: Soak the seeds with hormones, and soak the seeds in distilled water as a control. The treatment time is 3-5 hours, and then the seeds are planted in the greenhouse;

[0038] Step 2: Select the top leaves of female plants in the control (untreated) and treatment groups, freeze them quickly in liquid nitrogen, store them at -80°C, and prepare for metabolome analysis;

[0039] Step 3: Metabolite extraction and sample preparation:

[0040](1) Take 100 mg of the tissues of the control group and the treatment group in step 2, thaw and grind them respectively, place them in an EP tube, and add 500 μL of 80% methanol aqueous solution;

[0041] (2) Vortex, stand in an ice bath for 5 minutes, centrifuge at 15000g, 4°C for 20 minutes;

[0042] (3) Take a certain amount of supernatant and add mass spectrometry grade water to dil...

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Abstract

The invention discloses a method for mining industrial cannabis sativa hormone regulation response genes based on a metabonomics technology, and belongs to the technical field of industrial cannabis sativa differential metabolite identification. The method comprises the following steps: soaking seeds with hormone for 3-5 hours by taking distilled water seed soaking as a contrast, and then planting the seeds in a greenhouse; selecting top-end leaves of female plants in the contrast and treatment group in the flowering period, quickly freezing with liquid nitrogen, storing at-80 DEG C, and analyzing metabolome for later use; extracting a metabolite and preparing a sample; performing LC-MS analysis; carrying out data preprocessing and metabolite identification; and completing gene mining through mapping analysis and database query analysis. According to the invention, the influence of exogenous hormone on the different metabolites in the flowering phase of the Chinese hemp flowers is researched, detailed information is provided for deeply excavating metabolites, difference changes and the like which respond to hormone regulation in the flowering phase, and technical reference and theoretical support are provided for researches on regulation and control of growth and development, molecular breeding and the like by using a plant growth regulator in production.

Description

technical field [0001] The invention belongs to the technical field of identification of differential metabolites of industrial hemp, and in particular relates to a method for mining industrial hemp hormone-regulated response genes based on metabolomics technology. Background technique [0002] Industrial hemp has a long history of development in my country. In recent years, the annual planting area has reached about 1 million mu. China has become a big country in the cultivation, production, consumption and export of industrial hemp. A series of studies have proved that medical and health ingredients such as cannabinoids in the flowers and leaves of industrial hemp, seeds and the nutrients contained in them, fibers, rod cores and other raw materials have unique applications and effects in various fields of military and civilian. The interest and demand for products are increasing day by day, and the industrial hemp industry has become one of the characteristic economic indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/72G01N30/88
CPCG01N30/02G01N30/06G01N30/54G01N30/72G01N30/88G01N2030/8809
Inventor 王晓楠曹焜孙宇峰赵越王盼张治国朱浩孙凯旋
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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