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Yi medicine polygonum persicaria extract as well as preparation method and anti-cancer application thereof

A technology of Polygonum serrata extract, which is applied in the field of Yi drug Polygonum serrata extract and its preparation, can solve problems such as research lag, achieve the effects of improving resource development, promoting development and utilization, and simplifying the preparation process

Inactive Publication Date: 2021-11-02
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Polygonum serrata, as a national herbal medicine with traditional medicinal experience, has not been paid attention to, and its related research has lagged behind.
At present, there is no report about the application of Polygonum serrata in the preparation of anticancer drugs or health products

Method used

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  • Yi medicine polygonum persicaria extract as well as preparation method and anti-cancer application thereof
  • Yi medicine polygonum persicaria extract as well as preparation method and anti-cancer application thereof
  • Yi medicine polygonum persicaria extract as well as preparation method and anti-cancer application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of different polarity extraction parts of Polygonum serrata tuber root

[0039] (1) Powder the hard root tubers of Polygonum serrata after drying in the shade, pass through a 40-mesh sieve, add 95% ethanol solution according to the material ratio of 1:20 (g / mL), stir well, and then room temperature (20±5°C) ) conditions soaked. During the immersion process, the infiltrated samples were thoroughly stirred every 4 hours for a total of 24 hours.

[0040] (2) Leave the ethanol extract obtained in step (1) until the liquid is clear, then filter the extract by pressure suction filtration, and repeat the suction filtration step 3 times until there is no medicinal residue.

[0041] (3) Steps (1) and (2) were repeated, and the roots of Polygonum serrata were soaked and extracted for 3 to 4 times for 24 hours, and all the extracts were collected.

[0042] (4) Concentrate the ethanol extract collected in step (3) under reduced pressure (temperature 45±5°C, ...

Embodiment 2

[0046] Example 2 Application of different extraction parts of Polygonum serrata root tuber in research and development of anticancer drugs

[0047] (1) Principle of MTS assay for cell viability

[0048] MTS is a brand new MTT analog, its full name is 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium, it is a yellow color of dyes. The succinate dehydrogenase in the mitochondria of living cells can metabolize and reduce MTS to generate soluble formazan (Formazan) compounds, and the content of formazan can be measured with a microplate reader at 490nm. Under normal circumstances, the amount of formazan produced is directly proportional to the number of living cells, so the number of living cells can be inferred from the optical density OD value.

[0049] (2) Experimental method

[0050] 1) Cell inoculation: Prepare a single cell suspension with culture medium (DMEM or RMPI1640) containing 10% fetal bovine serum, and inoculate into a 96-well...

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Abstract

The invention belongs to the technical field of natural medicine extracts, particularly relates to a Yi medicine Polygonum serratum extract as well as a preparation method and anti-cancer application thereof, and aims to enhance the research on the application of Polygonum serratum in anti-cancer medicines and health-care products and improve the resource development and utilization of the Polygonum serratum which is a national medicinal plant. The invention provides an effective, stable and safe method for preparing different polarity extraction parts of polygonum persicaria root tubers. The extracted ethanol extract, the extracted ethyl acetate extract and the extracted n-butyl alcohol extract of the polygonum persicaria tubers show a certain inhibition effect on lung cancer cells, liver cancer cells, breast cancer cells, colon cancer cells and neuroblastic carcinoma cells. The extraction and preparation process of the anti-cancer effective part of the polygonum persicaria is simple, efficient, green and environmentally friendly, and further development of polygonum persicaria plant resources of polygonum multiflorum plants is facilitated.

Description

technical field [0001] The invention belongs to the technical field of medicine extracts, and in particular relates to an extract of Polygonum serrata, a Yi medicine, a preparation method thereof and an anticancer application. Background technique [0002] Cancer is an important disease that seriously endangers human health. According to statistics published in the World Cancer Report 2020 by the International Agency for Research on Cancer (IARC), there will be approximately 19.3 million new cases of cancer worldwide in 2020, and 10 million cancer deaths, of which the highest mortality rate from lung cancer is 18.0%. , followed by colon cancer (9.4%), liver cancer (8.3%), stomach cancer (7.7%) and breast cancer (6.9%). The next few years will be years of high incidence of cancer worldwide. If countries do not take urgent measures, by 2040, there will be 28.4 million new cancer cases worldwide every year. Therefore, the task of cancer prevention and treatment is very arduou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/704A23L33/105A61P35/00A61K125/00
CPCA61K36/704A61P35/00A23L33/105A61K2236/15A61K2236/333A61K2236/53A61K2236/51A23V2002/00A23V2200/308A23V2250/21
Inventor 薛永波苏向东邓文斌何鑫曾子谦
Owner SUN YAT SEN UNIV