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Process for preparing concentrated platelets through improved albuginea method

A platelet and buffy coat technology, applied in blood transfusion devices, suction devices, etc., can solve problems such as failure to meet quality requirements, waste of blood resources, low platelet recovery rate, etc., and achieve the effect of improving recovery rate and ensuring stability

Inactive Publication Date: 2021-11-02
株洲市中心血站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many factors affecting the preparation of PC at present, resulting in a low recovery rate of platelets, and may even fail to meet the quality requirements, resulting in a waste of blood resources

Method used

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  • Process for preparing concentrated platelets through improved albuginea method
  • Process for preparing concentrated platelets through improved albuginea method
  • Process for preparing concentrated platelets through improved albuginea method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The optimization of embodiment 1 centrifugation process

[0025] 180 bags of 400 mL whole blood were randomly divided into 9 groups. On the basis of the centrifugation parameters of the traditional buffy coat method, the relative centrifugal force was gradually adjusted, and 9 different centrifugation schemes were adopted. See Table 1 for details. PC is prepared by the modified buffy coat method: the raw whole blood is kept at a constant temperature of 22±2°C for 2 hours on average, centrifuged for 16 minutes, and the buffy coat is separated, and then the buffy coat is kept at a constant temperature of 22±2°C for 2 hours or 12 hours (generally It is said to stand still for 2 hours. In some cases, the main reason is that when the blood collection vehicle returns the blood sample at night, we usually first let it stand for 2 hours and then perform the first centrifugation to separate the buffy membrane, then let it stand for 12 hours, and perform the second in the next mor...

Embodiment 2

[0035] Embodiment 2 different whole blood volume groups

[0036] 30 bags of 300mL whole blood were prepared by the modified buffy coat method, and centrifugation scheme 6 was used to prepare PC, and the experimental data of PC prepared by centrifugation scheme 6 in 20 bags of 400mL whole blood were compared and analyzed. The results can be seen in Table 3,

[0037] Whole blood of 400mL and 300mL was used to prepare PC, and there was no statistically significant difference in platelet concentration between the groups before preparation (p>0.05). The recovery rate of PC prepared from 400mL whole blood was higher than that of 300mL whole blood, and the difference was statistically significant (p<0.05) See Table 3.

[0038] Table 3 Differences in the recovery rate of platelets prepared from whole blood with different blood volumes

[0039]

[0040] Note: compared with the 300mL whole blood group P a <0.05

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Abstract

The invention discloses a process for preparing concentrated platelets by an improved albuginea method, which comprises the following steps: 1) standing collected whole blood at constant temperature, centrifuging the whole blood after standing, and separating out albuginea rich in platelets; and 2) performing constant-temperature standing on the albuginea rich in the blood platelets in the step 1), centrifuging the albuginea rich in the blood platelets after standing, and separating supernate to obtain the blood platelets. According to the improved albuginea method, constant-temperature standing of whole blood is added on the basis of the albuginea method, and the principle is that platelets possibly reversibly aggregate in the blood transportation and handover process, depolymerization of the platelets is promoted by standing for a certain time, and therefore preparation and recovery of the platelets are facilitated. In order to prevent the blood bag from wrinkling, a special template is adopted for flat cup filling, the stability of the centrifugal process can be guaranteed, and the recovery rate of blood platelets can be increased.

Description

technical field [0001] The invention belongs to the technical field of blood sample processing, and in particular relates to a process for preparing concentrated platelets through an improved buffy coat method. Background technique [0002] With the continuous improvement of the level of clinical blood transfusion technology, the amount of platelet transfusion has increased significantly year by year. The application of platelets in most areas of my country is mainly based on apheresis platelets. Although apheresis platelets have the advantages of high purity, good clinical transfusion effect, and less white blood cell contamination, it is difficult to recruit blood donors and the cost of apheresis is high. Insufficient supply makes it difficult to meet the growing clinical demand. Concentrated platelets (Platelet concentrates, PC) are prepared by collecting and separating whole blood, which have the advantages of low cost and wide sources, and are still widely used by small...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/02
CPCA61M1/0281
Inventor 杨小罗熊志高王海燕刘昕晨殷应辉周英姿
Owner 株洲市中心血站