Gene polymorphism detection kit for guiding psychiatric medication

Abstract

The invention discloses a gene polymorphism detection kit for guiding psychiatric medication. The kit comprises a specific primer group and a fluorescent probe group, wherein the specific primer group and the fluorescent probe group are used for detecting the rs1065852 site of a CYP2D6 gene, the rs1414334 site of an HTR2C gene, the rs1800497 site of an ANKK1 gene, the rs762551 site of a CYP1A2 gene, the rs489693 site of an MC4R gene and the rs1799978 site of a DRD2 gene. According to the invention, the primer group and the fluorescent probe group are utilized, and the template DNA is specifically amplified by using a fluorescent PCR amplification technology, so that the polymorphism of a plurality of sites of a plurality of genes related to psychotropic drugs can be accurately detected at the same time; ARMS primer design is adopted, mismatched bases are introduced, the specificity of primer amplification is improved, the sensitivity is high, and accurate genotyping can be conducted on human template DNA at the lowest DNA initial amount of 10 ng; the detection speed is high and the efficiency is high: the PCR reaction only needs less than 1 hour to complete the amplification reaction, the cost is lower, the required reagent consumables are all clinically common reagents, the cost is lower, and the clinical popularization is convenient.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a gene polymorphism detection kit for guiding psychiatric medication. Background technique [0002] At present, the clinical treatment methods and methods of mental illness are still mainly based on drug treatment. Schizophrenia is one of the most serious mental illnesses. Schizophrenic patients generally need long-term or even life-long medication, that is, the patient's dependence on drugs and High viscosity. Therefore, while psychotropic drugs are used clinically to treat and save patients, the adverse reactions caused by them cannot be ignored. Clinical practice shows that in different stages of drug metabolism, transport, receptors, etc., when different individuals receive the same drug treatment, there are obvious differences in its therapeutic effect, side effects and drug tolerance. difference. Gene polymorphism is the essential cause of individual differences in d...

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Problems solved by technology

[0003] At present, the detection of genes related to psychotropic drugs mainly includes PCR-Sanger sequencing method, fluorescent quantitative PCR method, and gene chip method. Factors such as long cycle, low detection throughput, and high cost limit its rapid and accurate screening in clinical laboratories, so it is not suitable for the detection of multiple genes and multiple sites; and the gene chip method is due to high cost and complicated operation. and other factors limit its rapid and accurate screening in clinical laboratories; fluorescent quantitative PCR method has the advantages of high sensitivity, accurate typing, simple and fast operation, etc., and is currently widely used in clinical genotyping detection

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