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Active takifugu flavidus fish skin polypeptide as well as preparation method and application thereof

An active and fish-skin technology, applied in the field of peptides, can solve the problems affecting the elution efficiency and achieve the effect of fast and efficient screening, simple and easy operation, and good hypoglycemic effect

Pending Publication Date: 2021-11-05
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The currently prepared active chrysanthemum yellow oriental puffer fish skin polypeptide needs to be subjected to adsorption and elution treatment for the peptide components after enzymatic hydrolysis. Among them, the adsorption column plays a vital role, especially its packing, which affects the elution efficiency

Method used

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  • Active takifugu flavidus fish skin polypeptide as well as preparation method and application thereof
  • Active takifugu flavidus fish skin polypeptide as well as preparation method and application thereof
  • Active takifugu flavidus fish skin polypeptide as well as preparation method and application thereof

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Embodiment Construction

[0033] The present invention will be further described in conjunction with the accompanying drawings and specific embodiments.

[0034] The active chrysanthemum yellow oriental fugu skin polypeptide provided in this example is a decapeptide, and its amino acid sequence is TLRFALHGME.

[0035] This embodiment also provides a method for preparing active chrysanthemum yellow oriental puffer fish skin polypeptide, comprising the following steps:

[0036] S0: Preparation of immobilized ACE gel affinity adsorption column

[0037] ACE with an enzyme activity unit of 5 U / ml was dissolved in 50 ml of 0.1M borate buffer (pH 8.3, containing 0.5M NaCl) to prepare an ACE chelating ligand solution. Weigh 10g of cyanogen bromide-activated agarose 4B filler and put it in a 100ml beaker, add 50mL of 1mM HCl to swell, wash and filter with 0.1M borate buffer (pH 8.3, containing 0.5M NaCl) after filtration, and remove residual HCl. Cyanogen bromide-activated agarose 4B filler was obtained. Mix...

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Abstract

The invention discloses an active takifugu flavidus fish skin polypeptide as well as a preparation method and application thereof, and belongs to the technical field of polypeptides. The active takifugu flavidus fish skin polypeptide is decapeptide, and the amino acid sequence of the active takifugu flavidus fish skin polypeptide is TLRFALHGME. The preparation method of the active takifugu flavidus fish skin polypeptide comprises the following steps: S0, preparation of an immobilized ACE gel affinity adsorption column; S1, preparation of peptide components; S2, affinity adsorption and elution of the peptide components; S3, sequence identification of peptides; and S4, verification of ACE inhibitory activity of the compound active takifugu flavidus fish skin polypeptide. The prepared immobilized ACE gel affinity adsorption column is used for screening the active takifugu flavidus fish skin polypeptide, has the advantages of being rapid and efficient in screening and easy and convenient to operate, and can be used for rapid large-scale screening of the active takifugu flavidus fish skin polypeptide, and the active takifugu flavidus fish skin polypeptide has ACE inhibitory activity and DPP-IV inhibitory activity.

Description

technical field [0001] The invention belongs to the technical field of polypeptides, and in particular relates to an active chrysanthemum yellow oriental puffer fish skin polypeptide and a preparation method and application thereof. Background technique [0002] With the progress and development of society, people's living standards are gradually improving, and the proportion of fat and calories in the diet is gradually increasing. Coupled with the reduction in exercise, the incidence of cardiovascular diseases in my country has risen rapidly, of which hypertension is the main one. [0003] Under normal circumstances, the human body can ensure the balance between the source and outlet of blood sugar through the two major regulatory systems of hormone regulation and nerve regulation, so that blood sugar can be maintained at a certain level. However, under the joint action of genetic factors (such as family history of diabetes) and environmental factors (such as unreasonable d...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/22C12P21/06B01D15/20B01J20/24B01J20/28B01J20/30A61K38/08A61P9/12A61P3/10
CPCC07K7/06C12P21/06B01D15/206B01J20/24B01J20/28047A61P9/12A61P3/10A61K38/00Y02A40/81
Inventor 苏永昌刘智禹刘淑集陈贝乔琨许旻陈晓婷
Owner FISHERIES RES INST OF FUJIAN
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