Cell assembly method for Faraday wave multi-wavelength synthesis and application

A Faraday wave and assembly method technology, applied in animal cells, tumor/cancer cells, vertebrate cells, etc., to achieve dynamic adjustable patterns, huge application prospects, commercial transformation potential, and good biocompatibility

Active Publication Date: 2021-11-05
SHENZHEN CONVERGENCE BIO-MFG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The problem to be solved by the present invention is to provide a Faraday wave multi-wavelength synt...

Method used

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  • Cell assembly method for Faraday wave multi-wavelength synthesis and application
  • Cell assembly method for Faraday wave multi-wavelength synthesis and application
  • Cell assembly method for Faraday wave multi-wavelength synthesis and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Faraday wave multi-wavelength synthesis assembly for alignment of polystyrene microspheres

[0024] Add polystyrene microspheres into 0.01M phosphate buffer containing 0.05% Tween 20, and disperse fully. Polystyrene microspheres are red in color, with a diameter of 100 μm and a density of 1.05 g / cm 3, whose physical properties are similar to those of cell-containing assembly units, and the concentration of polystyrene microspheres is 30mg / mL. In this example, polystyrene microspheres were used to simulate cell-containing assembly units.

[0025] 1. Arrangement of polystyrene microspheres under single wavelength conditions, the steps are as follows:

[0026] After setting the waveform as a continuous sine wave, set the driving frequency as 20Hz, 28Hz, 58Hz, and the signal amplitude as 60-100mVpp, and output a specific electrical signal by adjusting the arbitrary waveform / function signal generator (Tecktronix, AFG3052C). The signal is transferred to the powe...

Embodiment 2

[0033] Example 2: Faraday wave multi-wavelength synthesis assembly for the arrangement of HepG2 cell microspheres

[0034] The steps for the arrangement of HepG2 cell microspheres under single wavelength conditions are as follows:

[0035] Add HepG2 cells into a six-well low-adhesion plate (Corning, 3471), 2*10 per well 6 cells, add 2 mL of DMEM complete medium. Place the six-well low-adhesion plate on a plate shaker with a rotation speed of 75 rpm, and cultivate for three days to form a ball. One well of HepG2 cell microspheres was collected and added to 0.01M phosphate buffer for use.

[0036] After setting the waveform as a continuous sine wave, set the driving frequency as 42Hz, 45Hz, and the signal amplitude as 60-80mVpp, and output a specific electrical signal by adjusting the arbitrary waveform / function signal generator (Tecktronix, AFG3052C), and the electrical signal passes through The BNC female to RCA male wire is transferred to the power amplifier (DAYTONAUDIO, ...

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Abstract

The invention discloses a biological assembly method for Faraday wave multi-wavelength synthesis and application. The method has the advantages that a system is easy to build, operation and control are simple, patterns are dynamically adjustable, biocompatibility is good and the like. The method is different from a cell manipulation principle under a single wavelength condition in an existing acoustic biological assembly method, sine or cosine signals with different wavelengths are synthesized, a single-wavelength assembly mode in a traditional Faraday wave frequency domain is improved into a multi-wavelength assembly mode, and complex and arbitrary pattern arrangement of liquid-bottom multi-scale cells is realized. The method is more suitable for the requirement for complex arrangement of cells in tissue engineering and biological manufacturing, and has huge application prospects and commercial value.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and biomanufacturing under biomedical engineering, and specifically relates to a biological assembly method and application of Faraday wave multi-wavelength synthesis. Background technique [0002] In human tissues and organs, cells connect to each other through self-organization and form highly ordered cell structures. The arrangement of cells and the geometry of the structure in the reduced structure in vitro not only play a key role in simulating the shape of tissues and organs in vivo at the macro scale, but also play an important role in regulating the microenvironment of cells. [0003] In recent years, biomanufacturing has shown great potential in constructing highly complex and functional cellular microstructures of human tissues and organs. Biomanufacturing is the use of bioprinting or bioassembly followed by tissue maturation to construct living cells, biomaterials, and stimu...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N5/00C12N5/09
CPCC12N13/00C12N5/00C12N5/0693C12N5/067C12N2527/00
Inventor 陈璞谷龙军
Owner SHENZHEN CONVERGENCE BIO-MFG CO LTD
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