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Method for rapidly and efficiently extracting macromolecular compound from sheep embryonic cells

A technology of sheep embryos and macromolecules, applied in the biological field, can solve the problems of inability to exert molecular weight active ingredients of sheep embryo cells, not containing macromolecular active ingredients, and reducing the active ingredients of extracts, so as to promote the growth of fetal fibroblasts and omit Enzyme reaction steps, reducing potency-reducing effects

Inactive Publication Date: 2021-11-09
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the currently published patented technology for extracting small-molecule sheep placenta from sheep embryo tissue cells is time-consuming, laborious, low extraction efficiency, and active ingredients of the extract are reduced, and does not contain macromolecular active ingredients above 6000Da, so it cannot Exert the functions of various molecular weight active ingredients in sheep embryonic cells

Method used

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Examples

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Effect test

Embodiment 1

[0031] A method for rapidly and efficiently extracting macromolecular complexes from sheep embryo cells, the method comprising the following steps:

[0032] (1) Treatment of sheep fetuses: aseptically remove 2-month-old fetuses from the sheep uterus, wash the fetuses with sterile ultrapure water for 3 times, separate the whole body muscle tissue of the fetuses with a sharp knife and cut them into small pieces, wash them with sterile ultrapure water Wash the muscle tissue 3 times to remove blood and other impurities. The separated tissue is preliminarily crushed by a grinder and then put into a stainless steel container. The container is placed on the liquid nitrogen liquid surface for quick freezing for 20 minutes, and then dried at low temperature;

[0033] (2) Dry the sample at 4°C for low-temperature ultrafine pulverization, pass through a 150-mesh sieve to obtain the original powder, and mix with 1 volume of pre-cooled sterile ultrapure water to obtain the original solution...

Embodiment 2

[0041] A method for rapidly and efficiently extracting macromolecular complexes from sheep embryo cells, the method comprising the following steps:

[0042] (1) Treatment of sheep fetuses: aseptically remove 4-month-old fetuses from the sheep uterus, rinse the fetuses with sterile ultrapure water for 3 times, separate the whole body muscle tissue of the fetuses with a sharp knife and cut them into small pieces, wash them with sterile ultrapure water Wash the muscle tissue 3 times to remove blood and other impurities. The separated tissue is preliminarily crushed by a grinder and then put into a stainless steel container. The container is placed on the liquid nitrogen liquid surface for quick freezing for 20 minutes, and then dried at low temperature;

[0043] (2) Dry the sample at 4°C for low-temperature ultrafine pulverization, pass through a 300-mesh sieve to obtain the original powder, and mix and stir with 4 times the volume of pre-cooled sterile ultrapure water to obtain t...

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Abstract

The invention relates to a method for quickly and efficiently extracting a macromolecular compound from sheep embryonic cells. The method mainly comprises two parts of contents. The core technical content I is that: fetal tissue cells are quick-frozen by liquid nitrogen, the obtained product is dried, and the obtained product is subjected to primary treatment by using a low-temperature ultrafine grinder; and the core technical content II is that: tissue cell precipitates and molecular complexes are gradually separated by using a method of carrying out centrifugation with different centrifugal speeds, and molecular peptide substances in cells are released to the maximum extent by combining ultrahigh-pressure cell crusher and low-temperature ultrasonic cell crusher treatment technologies. By the method, compound sheep placenta containing macromolecular and micromolecular peptides and the like can be obtained, natural active ingredients in the sheep embryonic cells are reserved to the maximum extent, the whole operation needs short time and is carried out under a low-temperature condition, the influence of temperature and time on the active ingredients such as the micromolecular peptide and the like in the extraction process is guaranteed to the maximum extent, and no any chemical reagent is added in the extraction process, so that the product obtained in the invention has better biological functions than a product obtained in the prior art.

Description

technical field [0001] The invention relates to a method for rapidly and efficiently extracting macromolecular complexes from sheep embryo cells, belonging to the field of biotechnology. [0002] technical background [0003] Sheep embryonic tissue cells are rich in biological macromolecules, small molecular peptides, various amino acids, hyaluronic acid stimulating factors, superoxide dismutase and other biologically active ingredients, which have significant anti-aging, anti-oxidation, and anti-fatigue effects , Improving the body's immunity and so on. Small molecule sheep placenta extracted from sheep embryo cells has been widely used in cosmetics, health care products and other fields. [0004] In the currently published patented method for extracting small-molecule sheep placenta, the 3-month-old fetus used only removes internal organs, head, etc., and the fetus contains a large amount of cartilage tissue such as unformed bone tissue. Materials containing a large amount...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073A61K8/98A61Q19/00A61Q19/08
CPCC12N5/0603A61K8/982A61Q19/00A61Q19/08C12N2509/10A61K2800/84A61K2800/805
Inventor 袁玉国钟平蔡何清李玲王佳琳
Owner YANGZHOU UNIV
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