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Preparation for improving activity and/or thermal stability of superoxide dismutase and application of preparation

A technology of superoxide and thermal stability, applied in the biological field, can solve the problems of hindering popularization and application, poor thermal stability, low SOD activity, etc., and achieve the effect of improving the safety of use

Pending Publication Date: 2021-11-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, SOD itself has the disadvantages of low activity and poor thermal stability, which hinder its popularization and application.

Method used

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  • Preparation for improving activity and/or thermal stability of superoxide dismutase and application of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In this example, the effect of different small molecular substances on superoxide dismutase was studied.

[0021] 1. Reagents

[0022] Catechin solution: Weigh 2.9 mg catechin and dissolve it in 10 mL of ethanol to obtain C 儿茶素 =1×10 -3 mol / L stock solution

[0023] Quercetin solution: Weigh 3.0mg of quercetin and dissolve in 10mL of ethanol to obtain C 槲皮素 =1×10 -3 mol / L stock solution

[0024] Myricetin solution: weigh 3.2mg myricetin and dissolve it in 10mL of ethanol to obtain C 杨梅素 =1×10 -3 mol / L stock solution

[0025] Kaempferol solution: Weigh 2.9mg kaempferol and dissolve it in 10mL of ethanol to obtain C 山奈素 =1×10 -3 mol / L stock solution

[0026] Ellagic acid solution: weigh 3.0 mg ellagic acid and dissolve it in 10 mL of ethanol to obtain C 鞣花酸 =1×10 -3 mol / L stock solution

[0027] Ferulic acid solution: Weigh 1.9mg of ferulic acid and dissolve it in 10mL of ethanol to obtain C 阿魏酸 =1×10 -3 mol / L stock solution

[0028] VB1 solution: Weigh 3....

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Abstract

The invention provides a preparation for improving activity and / or thermal stability of superoxide dismutase and an application of the preparation. The preparation comprises at least one of catechin, quercetin, myricetin, kaempferol, ellagic acid and ferulic acid. The quercetin, the myricetin and / or the kaempferol contained in the preparation can effectively improve the activity and the thermal stability of the superoxide dismutase, and the superoxide dismutase is endowed with a relatively wide application prospect.

Description

technical field [0001] The present invention relates to the field of biology. In particular, the present invention relates to preparations for increasing the activity and / or thermostability of superoxide dismutase and applications thereof. Background technique [0002] Superoxide dismutase (Superoxide dismutase, SOD) is a kind of redox metalloenzyme, which can remove superoxide free radical anions in the body and effectively prevent the damage of superoxide free radical anions to the body. It is the number one source of oxygen free radicals in the body. Killers are the foundation of life and health. SOD enzymes are widely distributed in various organisms, such as animals, plants, microorganisms, etc. However, SOD itself has the disadvantages of low activity and poor thermal stability, which hinder its popularization and application. Contents of the invention [0003] The present invention aims to solve at least one of the technical problems existing in the prior art at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K47/22A61K47/12A61P39/06A23L33/105
CPCA61P39/06A23L33/105A23V2002/00A61K38/446C12Y115/01001A61K47/22A61K47/12A23V2200/30A23V2250/2132A23V2250/214A23V2250/21168A23V2250/2116A23V2250/21A23L33/15C12N9/0089
Inventor 廖小军赵阳王永涛赵靓饶雷
Owner CHINA AGRI UNIV
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