Gene sequencing method and gene sequencer

A technology of gene sequencer and communication direction, applied in the field of gene sequencing method and gene sequencer, can solve the problems of lengthening the detection process and reducing the reliability of the system, etc.

Inactive Publication Date: 2021-11-12
BEIJING HAIWEIER TECH DEV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above mechanical movement will lengthen the inspe

Method used

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  • Gene sequencing method and gene sequencer
  • Gene sequencing method and gene sequencer
  • Gene sequencing method and gene sequencer

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Embodiment Construction

[0038] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application.

[0039] At present, some genetic detectors need to mechanically switch the optical path during the detection process, and mechanical movement will lengthen the detection process and reduce system reliability.

[0040] In order to improve the reliability of the gene sequencer and increase the detection speed, the present application provides a gene sequencer.

[0041] refer to figure 1 , the gene sequencer includes an excitation optical path, an imaging optical path and a focusing optical path.

[0042] The excitation light path is used to transmit the excitation light beam generated by the excitation light source 1 to the fluid chip 15 having a flow channel...

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Abstract

An embodiment of the invention provides a gene sequencing method and a gene sequencer, and relates to the technical field of gene sequencing. The gene sequencer comprises an excitation light path used for conducting an excitation light beam generated by an excitation light source to a fluid chip with a flow channel, wherein a sample to be tested flows through the flow channel; an imaging light path which is used for collecting fluorescent light generated by the object to be detected through excitation of the excitation light beam and imaging the fluorescent light on an imaging camera arranged on the imaging light path; and a focusing light path used for transmitting a focusing light beam generated by the focusing light source to the fluid chip; and objective lenses are arranged on the excitation light path, the imaging light path and the focusing light path, the objective lenses are located above the fluid chip and can move to change the distance between the objective lenses and the fluid chip, and the focusing light beam and the excitation light beam are focused on the upper surface or the lower surface of the flow channel by changing the distance between the objective lenses and the fluid chip. The reliability of the gene sequencer can be improved, and the detection speed is increased.

Description

technical field [0001] The present application relates to the technical field of gene sequencing, in particular to a gene sequencing method and a gene sequencer. Background technique [0002] At present, when using the fluorescence detection method to sequence genes, some products use a line-scan camera as a detector, and use a motor to drive the microfluidic chip to scan while moving. It will distort the image and affect the test accuracy. Other products use an area scan camera as a detector, detecting different colors requires turning the filter wheel, and the objective lens focusing system needs to switch mirrors. The above-mentioned mechanical movement device will lengthen the detection process and reduce the reliability of the system. Contents of the invention [0003] In order to improve the reliability of a gene sequencer and increase the detection speed, the present application provides a gene sequencer method and a gene sequencer. [0004] In the first aspect, ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C12Q1/6869
CPCG01N21/6428G01N21/6456C12Q1/6869G01N2021/6439
Inventor 张哲夫
Owner BEIJING HAIWEIER TECH DEV
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