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Preparation method and reaction device of microcarrier suitable for three-dimensional cell culture

A technology for preparing a device and a liquid, which is applied in the field of preparation of microcarriers and can solve the problems of limited production volume of production methods and the like

Pending Publication Date: 2021-11-16
BEIJING CYTONICHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the large market demand for the microslide, and the limited production capacity of the existing production methods, it is urgent to adopt new methods and equipment to expand the production scale

Method used

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  • Preparation method and reaction device of microcarrier suitable for three-dimensional cell culture
  • Preparation method and reaction device of microcarrier suitable for three-dimensional cell culture
  • Preparation method and reaction device of microcarrier suitable for three-dimensional cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0139] Oil ratio

[0140] 1.1 First check the cleanliness of the mixing tank, pre-cooling tank, liquid sterilizing filter, measuring tools and site clearing records. They are clean and can be used within the clearing period;

[0141] 1.2 Take 100L of petroleum ether and 10L of PO-500 reagent into the mixing tank sequentially using a peristaltic pump. Open the mixing tank and stir, and the rotating speed is set at 60rpm to stir for 1 hour;

[0142] 1.3 Close the tank body of the mixing tank and only keep the compressed air port open, until the internal pressure of the tank body reaches 0.1MP, pour the oil phase into the pre-cooling tank. Through the continuous cooling of the chiller, the temperature of the oil phase is reduced to -10°C for standby, and the agitation of the pre-cooling tank can be kept at 40rpm during this period.

[0143] water ratio

[0144] 2.1 First check the cleanliness of the water phase tank, measuring tools and site clearance records, and they can be ...

Embodiment 2

[0164] Oil ratio

[0165] 1.1 Inspection device, same as embodiment 1;

[0166] 1.2 Measure 80L of chloroform, 20L of petroleum ether, and 10L of Tween 20 reagent and add them to the mixing tank sequentially using a peristaltic pump. Open the mixing tank and stir, and the rotating speed is set at 60rpm to stir for 1 hour;

[0167] 1.3 Close the tank body of the mixing tank and only keep the compressed air port open, until the internal pressure of the tank body reaches 0.1MP, pour the oil phase into the pre-cooling tank. Through the continuous cooling of the chiller, the temperature of the oil phase is reduced to -30°C for standby, and the agitation of the pre-cooling tank can be kept at 40rpm during this period.

[0168] water ratio

[0169] 2.1 Inspection device, same as embodiment 1;

[0170] 2.2 Weigh 50g of gelatin + 10g of sodium alginate and put it into 1.5L deionized water. Set the agitator at 60rpm to stir the gelatin solution until it is fully dissolved. Use a peris...

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Abstract

The invention provides a method for preparing microcarrier particles. The method comprises that a dispersed phase liquid passes through a porous plate at a low temperature to form liquid microspheres in a continuous phase, and an artificially synthetic polymer and / or a natural biomacromolecule in the liquid microspheres is subjected to a curing reaction at a low temperature to form a particulate matter. The invention further provides a method for preparing emulsion, and a device and process system for preparing microcarrier particles, which can be used for large-scale preparation of emulsions , microcarrier particles.

Description

technical field [0001] The present disclosure relates to a method and a device for preparing microcarriers, especially a method and a process system for large-scale preparation of microcarriers. Background technique [0002] 3D TableTrix Microslide (Beijing Huakan Biotechnology Co., Ltd.) is a new type of cell expansion carrier material customized for stem cell production. The technology product adopts innovative microcarrier tablet design, each microcarrier has clear weight, independent sterilization, ready-to-use, eliminating the cumbersome operations required by traditional microcarriers such as weighing and sterilization, and is superior to traditional microcarriers. Vector for cell culture. [0003] Microcarriers can be prepared in the form of microslides. When the micro-slide meets water, it will disperse into tens of thousands of elastic three-dimensional porous micro-carrier particles, and the 3D structure and physical properties of the dispersed elastic micro-carr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08L89/00C08L5/08C12N5/00
CPCC08J9/286C08J9/0061C12N5/0062C12N5/0075C12N2513/00C12N2531/00C08J2389/00C08J2405/08C08J2201/0484C08L89/06C08J9/283C08J2201/026C08J2389/06C08J2405/04B01F23/41B01F25/31422C08L5/04B01F23/4143B01F23/4145B01F23/4105B01F23/808B01F2101/44C08J9/28C08L89/00C12N2533/54C12N2533/74C09K23/00
Inventor 刘伟鄢晓君
Owner BEIJING CYTONICHE BIOTECH CO LTD
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