Paper-based electrochemical luminescence nucleic acid instant detection chip as well as preparation method and application thereof

Abstract

The invention discloses a paper-based electrochemical luminescence nucleic acid instant detection chip as well as a preparation method and application thereof. The detection chip comprises a first sealing film layer, a paper channel layer, an electrode layer and a second sealing film layer which are distributed from top to bottom, the paper channel layer comprises an inverted E-shaped wax printing paper-based microchannel consisting of a first channel and a second channel, a first end of the first channel is a sample adding area, a detection area is arranged on the first channel, an electrochemical luminescence reagent is fixed in the detection area, and a second end of the first channel is connected with the second channel; the electrode layer comprises three electrodes, and the three-electrode area corresponds to the detection area in position; and the first sealing film layer is provided with a sample adding hole corresponding to the sample adding area. The nucleic acid detection chip is a label-free, simple and universal paper-based instant detection chip and can be widely applied to the field of medical detection.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a paper-based electrochemiluminescence nucleic acid real-time detection chip and its preparation method and application. Background technique [0002] The World Health Organization (WHO) has proposed the POCT (Point of Care Testing, POCT) ASSURED principle for point of care testing in low- and middle-income countries, which can be paid ( Affordable), specific (Sensitive), sensitive (Specific), user-friendly (User friendly, easy to complete within a few steps of minimum training), rapid and reliable detection (Rapid and Robust, the results can be obtained within 30 minutes of reagent storage at room temperature), no equipment or equipment Simple (Equipment free or minimal equipment), direct delivery (deliverable to end-users). At present, the entire nucleic acid real-time detection field is in the early stage of market development. Domestic and foreign real-time molecular detecti...

AI Technical Summary

Problems solved by technology

[0003] Traditional nucleic acid detection methods mainly use fluorescent PCR (polymerase chain reaction) technology, but limited by many factors such as equipment, electricity, space, and operating technicians, bedside diagnosis cannot be effectively realized

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