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Biological target digital quantitative detection system based on virtual segmentation method

A biological target, quantitative detection technology, applied in biological testing, chemiluminescence/bioluminescence, measurement devices, etc., can solve the problems of uneven spatial size of reaction units, non-independent reaction units, low number of reaction units, etc. Reduced costs of consumables and detection systems, high elution and reaction efficiencies, and broadened applications

Active Publication Date: 2021-11-23
IOMICS BIOSCIENCES INC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] (2) The size of the reaction unit space is uniform and the distribution is random
[0015] The reaction units are not independent of each other, the spatial size of the reaction units is not uniform, and the number of reaction units is too low, which will cause errors in the detection of downstream results

Method used

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  • Biological target digital quantitative detection system based on virtual segmentation method
  • Biological target digital quantitative detection system based on virtual segmentation method
  • Biological target digital quantitative detection system based on virtual segmentation method

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Embodiment

[0043] Example Digital quantitative detection system for biological targets based on virtual segmentation method

[0044] A digital quantitative detection system for biological targets based on virtual segmentation method such as figure 1 shown, including the following devices:

[0045] 1. Sample processing and enrichment device

[0046] Such as figure 2 with image 3 as shown, figure 2 It is a schematic diagram of the principle of separately capturing multiple DNA / protein target molecules from a biological sample; and image 3 It is a schematic diagram of the principle of separately capturing DNA / protein target molecules from multiple biological samples. Use micro-nano magnetic beads to process and enrich human liquid samples (blood, body fluids, tissues, etc.) to capture DNA / protein target molecules to be tested. First, the surface of the magnetic bead is modified with a specific ligand (nucleic acid, protein) linked to the DNA / protein target molecule to be tested. ...

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Abstract

The invention provides a biological target digital quantitative detection system based on a virtual segmentation method. The system comprises: a sample processing and enriching device for processing, enriching and capturing a to-be-detected biological target by using magnetic beads; a micro-fluidic detection chip, wherein the liquid of the to-be-detected biological target and an intermediate ligand reaction liquid respectively enter the micro-fluidic chip under the driving of pressure, and in the micro-fluidic chip, the to-be-detected biological target connected to the magnetic beads is combined with the intermediate ligand; and a detection device used for obtaining a digital picture on the reaction area plane of the micro-fluidic chip after the reaction, wherein digital quantitative detection of the to-be-detected biological target is realized by adopting a virtual segmentation method. According to the invention, the whole detection system is greatly simplified, detection consumables and the cost of the detection system are greatly reduced, and the application of the digital quantification technology is greatly widened.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a digital quantitative detection system for biological targets based on a virtual segmentation method. Background technique [0002] In Vitro Diagnostics (In Vitro Diagnosis, IVD) is a technology that obtains clinical diagnostic information by performing sample processing, biochemical reactions, and result detection on human samples (blood, body fluids, tissues, etc.) outside the human body. The detection object of in vitro diagnostic technology is liquid, and the routine detection volume is 1-100ml. The biological and chemical substances in the liquid are mainly nucleic acid molecules (DNA / RNA) and protein molecules. The primary human sample for in vitro diagnostic testing is blood. Since the concentration of biological and chemical substances in the normal human body is relatively constant in the blood, changes in the concentration of specific biological and chemical subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/76
CPCG01N33/5304G01N21/76Y02A50/30
Inventor 荆高山王栋许俊泉
Owner IOMICS BIOSCIENCES INC