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Application of small molecule inhibitor combination based on PNPT1 in preparation of antiviral drug

A technology of antiviral drugs and compositions, applied in the direction of antiviral agents, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effects of inhibiting virus replication, clinical drug safety, and reducing drug concentration

Pending Publication Date: 2021-11-30
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the antiviral activity of lanatoside C

Method used

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  • Application of small molecule inhibitor combination based on PNPT1 in preparation of antiviral drug
  • Application of small molecule inhibitor combination based on PNPT1 in preparation of antiviral drug
  • Application of small molecule inhibitor combination based on PNPT1 in preparation of antiviral drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 5

[0033] Example 1 An example of pentagalloyl glucose and lanatoside against adenovirus infection.

[0034] The shape of the adenovirus virus particle is a three-dimensionally symmetrical icosahedron with a diameter of 70-90 nanometers and no envelope. The outer layer is composed of 252 capsids, of which 240 are hexons, and the other 12 are called hexons. Pentons are located at the top of the 12 virions. The adenovirus genome is a linear dsDNA (double-stranded DNA), which is combined with internal proteins to form the core. In this study, the pAd-5 / eGFP plasmid (Stratagene, US) containing the genome sequence of adenovirus was constructed, and the virus was collected and purified after transfection into 293T cells (Hanbio Technology (Shanghai) Co., Ltd.).

[0035] The specific steps of the experiment are:

[0036] (1) Virus infection and drug treatment: use adenovirus to infect human lung adenocarcinoma cell line A549 at MOI=0.1, remove the medium containing the virus after 3 h...

Embodiment 2 5

[0042] Example 2 Pentagalloylglucose and lanatoside C are examples of anti-coronavirus infection in mice.

[0043]Mouse hepatitis coronavirus (MHV-A59) belongs to the genus Coronaviridae, and is a single-stranded RNA virus. As the prototype species of betacoronavirus, MHV-A59 is an ideal model for the study of the molecular mechanism and pathogenicity of coronaviruses. In this study, the pMH54-MHV-A59 plasmid (Stratagene, US) containing the MHV-A59 viral genome sequence was constructed, and the virus was collected and purified after transfection into L2 cells (Hanbio Technology (Shanghai) Co., Ltd.).

[0044] The specific steps of the experiment are:

[0045] (1) Virus infection and drug treatment: use mouse hepatitis coronavirus to infect the mouse nerve cell line Nero-2A at MOI=0.1, remove the medium containing the virus after 3 hours, wash it twice with PBS, add five different concentrations of Galloylglucose (0, 10, 25, 50, 100 μM) or culture medium containing different ...

Embodiment 3 5

[0051] Example 3 Pentagalloyl glucose and lanatoside C inhibit the replication of the new coronavirus S protein.

[0052] The novel coronavirus (COVID-19) is an enveloped virus composed of four structural proteins: spike (S) protein, membrane (M) protein, envelope (E) protein and nucleocapsid (N) protein. Among them, the S protein is responsible for the virus to attach and enter the target cell, thereby initiating the infection process. During SARS-CoV infection, the S protein plays a key role in inducing protective humoral and cellular immunity. Therefore, the S protein is considered to be the most effective therapeutic target in the treatment of new coronaviruses. In this study, the pFV-CoV-S plasmid containing the genome sequence of the COVID-19S protein was constructed and transfected into 293T cells to collect and purify the virus (Beijing Qingke Biotechnology Co., Ltd.).

[0053] The specific steps of the experiment are:

[0054] (4) Virus infection and administration...

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Abstract

The invention discloses application of a small molecule inhibitor combination based on PNPT1 in preparation of an antiviral drug. The antiviral drug combination takes pentagalloylglucose and verbascoside as effective components. The pentagalloylglucose and the verbascoside can obviously inhibit the replication capacity of DNA and RNA viruses when being used independently or jointly, and have extremely important clinical application potential and value.

Description

technical field [0001] The invention belongs to the field of new application of compounds, and relates to the application of a PNPT1-based small molecule inhibitor combination in the preparation of antiviral drugs. Background technique [0002] Viruses are usually composed of nucleic acid and protein shells, and some viruses also have an envelope structure. According to the types of nucleic acids contained in different viruses, the ways of transcription and protein synthesis are further divided into DNA viruses, RNA viruses and retroviruses. Viruses are granular non-cellular structures, usually in nanometers, that require parasitic life. After the virus invades the host cell, it uses the host cell to provide various biological raw materials such as nucleotides, amino acids, ribosomes and energy systems, etc., with the viral nucleic acid as the control center, to synthesize the nucleic acid and protein components required by the progeny virus, and finally in the The host ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/7024A61P31/14
CPCA61K31/7048A61K31/7024A61P31/14A61K2300/00Y02A50/30
Inventor 曾科梁宏伟渠爽黄海
Owner CHINA PHARM UNIV
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