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PDX model of low-risk acute myeloid leukemia CIT and construction method of PDX model
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A technology of myeloid leukemia and acute myeloid leukemia, applied in the field of PDX model and construction of low-risk acute myeloid leukemia CIT, can solve the problems of thrombocytopenia, bone marrow suppression, granulocytosis, etc., and achieve long survival time and stable model , Induce the effect of fast time
Pending Publication Date: 2021-12-03
ZHONGNAN HOSPITAL OF WUHAN UNIV
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Problems solved by technology
The 7+3 classic chemotherapyregimen has significantly improved the survival of many patients, but after chemotherapy, patients usually suffer from myelosuppression, varying degrees of neutropenia and thrombocytopenia, etc.
We found in our clinical work that the emergence of CIT may lead to lower doses of chemotherapy regimens, delayed treatment, weakened efficacy, and increased economic burden on patients
There is no report on building a PDX model for AML CIT
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Embodiment 1
[0062] A method for constructing a PDX model of low-risk acute myeloid leukemia CIT of the present invention, said method comprising:
[0063] Step S1, obtaining a PDX model of low-risk acute myeloid leukemia mice; specifically:
[0064] 1. Select the appropriate clinical patients:
[0065] (1) Inclusion criteria
[0066] a) Age ≥ 18 years old;
[0067] b) Cell smears and cytochemical examinations of peripheral blood and bone marrow meet the diagnostic criteria for acute myeloid leukemia No. 26, 36, and 37 issued by FBA and WHO; and there are flow cytometry, chromosome, and genetic tests The results suggest that patients with low-risk AML have a good prognosis;
[0068] c) The ECOG behavioral status score is 0 to 2 points;
[0069] d) Liver and kidney functions are basically normal: blood bilirubin ≤ 34.2 μmol / L, AST / ALT below 2 times the upper limit of normal value, serum creatinine ≤ 222 μmol / L; normal heart function: EF>50%; lung function: indoor oxygen Saturation ≥ 95...
experiment example 1
[0105] Implementation group 1: 6 B-NSG mice were used to conduct experiments using the method of Example 1 to form implementation group 1;
[0106] Contrast group 1: use 4 B-NSG mice to carry out experiments using the method of comparative example 1 to form a comparative group 1;
[0107] Contrast group 2: use 4 B-NSG mice to carry out experiments using the method of comparative example 2 to form a comparative group 2;
[0108] Contrast group 3: 8 B-NSG mice were used to conduct experiments using the method of comparative example 3 to form a comparative group 3;
[0109] The implementation group and the comparison group 1-3 respectively record the date of using cytarabine on the mice as 1d, and the subsequent 4th day, 7th day and 14th day are respectively recorded as 4d, 7d and 14d, and the CIT is carried out. Detection, the results are shown in Table 1;
[0112] Note: The basal value refers to the basal value of the p...
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Abstract
The invention discloses a PDX model of low-risk acute myeloid leukemia CIT and a construction method of the PDX model. The method comprises the following steps: obtaining a PDX model of a low-risk acute myeloid leukemia mouse; and injecting cytarabine into the mouse intraperitoneal cavity of the PDX model of the low-risk acute myeloid leukemia to induce thrombocytopenia caused by chemotherapy so as to obtain the PDX model of the low-risk acute myeloid leukemia CIT. According to the invention, the PDX model of the low-risk acute myeloid leukemia CIT is constructed for the first time, so that the real situation of a patient with the leukemia CIT can be better reflected, and an important basis is provided for scientific research of the leukemia CIT; and after CIT is induced by the model, the survival time is relatively long, the induction speed is high, and the model is stable.
Description
technical field [0001] The invention relates to the technical field of animal models, in particular to a PDX model of low-risk acute myeloid leukemia CIT and a construction method thereof. Background technique [0002] Acute myeloid leukemia (acute myelocytic leukemia, AML) is the most common acute leukemia in adults. It can be divided into 8 types in clinical practice, from M0 to M7. At present, in China, the prognosis risk of AML is mainly determined according to the changes of leukemia cytogenetics and molecular genetics at the time of first diagnosis. When cytogenetic test results are inv(16)(p13q22) or t(16;16)(p13;q22), with NPM1 mutations detected by molecular genetics but not FLT3-ITD mutations; or cytogenetic test results t (8; 21) (q22; q22), with CEBPA double mutations detected by molecular genetics, the prognosis of patients is good, and the risk of prognosis is low, so these patients are diagnosed as low-risk AML. The 7+3 classic chemotherapyregimen has signi...
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