Unlock instant, AI-driven research and patent intelligence for your innovation.

High-throughput drug screening system and method

A high-throughput, drug-based technology, applied in biochemical equipment and methods, chemical instruments and methods, biomass post-processing, etc., can solve the problems of application limitations, lower liquid flow velocity, and low efficiency of concentration gradient generation, and achieve The effect of reducing the number of layers of the flow channel structure, increasing the fluid flow rate, and improving the generation efficiency

Inactive Publication Date: 2021-12-03
SUZHOU HEALTH COLLEGE
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] First: The microfluidic chips in the above patents can generate concentration gradients. Combining these chips can be used to study the effects of drugs with different concentrations, but they cannot simultaneously screen the effects of different drugs, and their application has certain limitations.
[0007] Second: The concentration gradient generation structure in the above patent has some defects: on the one hand, it realizes the formation of multiple concentration gradients through multiple "Christmas tree" layers, and each additional layer will increase 1-2 streams in the layer Channel units, thus increasing 1-2 concentration gradients, for example, the first layer is generally 3 channel units, thus having 3 concentrations, and the second layer is generally 4-5 channel units, thus having 4-5 concentrations , increase the number of concentration gradients by increasing flow channel units, but the principle of its structure is to add several concentrations by mixing several solutions that cannot be concentrated in the upper layer. In order to ensure a more uniform concentration gradient, the increased flow channels in each layer Units cannot exceed a certain number, so to obtain more concentration gradients, more layers will be required
This leads to lengthy flow channels and low concentration gradient generation efficiency
On the other hand, each flow channel unit usually adopts a circuitous channel structure (such as an S-shaped channel structure) to achieve the mixing of the two liquids. The number of layers is high and the flow channel units are many, resulting in the entire flow channel There are many circuitous and curved flow channel structures in the structure, which will greatly increase the flow channel resistance and double the length of the flow channel, which will further reduce the liquid flow velocity and reduce the formation efficiency of the concentration gradient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-throughput drug screening system and method
  • High-throughput drug screening system and method
  • High-throughput drug screening system and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] refer to Figure 1-2 , a high-throughput drug screening system of the present embodiment, comprising:

[0085] The microfluidic chip 1 has a microfluidic channel structure 2 on it, and the microfluidic channel structure 2 includes a drug primary screening structure 20 and a drug fine screening structure 21, and the drug primary screening structure 20 is used to screen out pairs of drugs from different types of drugs. Candidate drugs with effects on target cells, the fine drug screening structure 21 is used to analyze and judge the effects of candidate drugs at different concentrations;

[0086] Optical detection module 7, which is used for optical detection of the reaction system in the drug primary screening structure 20 and the drug fine screening structure 21, to judge the effect of the drug;

[0087] The cultivation module is used to provide the required cultivation environment for the reaction system in the primary drug screening structure 20 and the fine drug scr...

Embodiment 2

[0112] refer to Figure 2-7 , as a further improvement on the basis of the above embodiment, in this embodiment, the fine drug screening structure 21 also includes a mixing unit 4 arranged between the concentration gradient generation unit 3 and the cell culture unit 5;

[0113] The concentration gradient generating unit 3 includes a gradient generating structure 30 and a concentration configuration structure 3131. The gradient generating structure 30 includes a selected drug inlet 300, a first distribution channel group 302 communicating with the selected drug inlet 300, and a channel group 302 connected to the first configuration. Several second distribution channel groups 304 connected by channel groups;

[0114] The first distribution channel group 302 includes N first distribution channels 3020 arranged at intervals along the Y direction, the first distribution channels 3020 are parallel to the X direction, and the lengths of all the first distribution channels 3020 are e...

Embodiment 3

[0150] refer to Figure 2-9 In this embodiment, N=3, M=3 (that is, the number of first distribution channels 3020 is 3, the number of second distribution channels 3040 is 9, and 9 concentration gradients can be generated) as a specific example for description.

[0151] In this embodiment, the width range of the channels in the first distribution channel group 302 is 50-1500 μm, and the depth range is 2-20 μm; the width range of the channels in the second distribution channel 3040 is 30-200 μm, and the depth range is 2-20 μm. 25 μm.

[0152] In one embodiment, the numbers are sequentially numbered from top to bottom, and the volumes of the 1st to 3rd first distribution channels 3020 are U 0 , 3U 0 、9U 0 , the volumes of the three second distribution channels 3040 connected after each first distribution channel 3020 are V from top to bottom 0 , 1.5V 0 , 2V 0 , that is, the volume ratio of the three second distribution channels 3040 in each second distribution channel group...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a high-throughput drug screening system and method. The system comprises a micro-fluidic chip, an optical detection module, a culture module and a liquid path module, wherein a micro-channel structure is arranged on the micro-fluidic chip, the micro-channel structure comprises a drug primary screening structure and a drug fine screening structure, the drug primary screening structure is used for screening out candidate drugs with effects on target cells from different types of drugs, and the drug fine screening structure is used for analyzing and judging the effects of the candidate drugs with different concentrations. The high-throughput drug screening system is based on the micro-fluidic chip, can perform high-throughput primary selection on a large number of drugs and screen out candidate drugs with specific effects on target cells, and can further generate a large number of candidate drug solutions with different concentrations and research the relationship between the concentrations of the candidate drugs and the effects of the candidate drugs so as to obtain the optimal concentration of the drugs.

Description

technical field [0001] The invention relates to the field of drug screening, in particular to a high-throughput drug screening system and method. Background technique [0002] In the process of new drug development, drug screening is used to adopt appropriate methods for substances that may be used as drugs to detect their possible pharmacological activities, so as to provide experimental data for the development of new drugs. Appropriate drug screening strategies can improve the efficiency of screening and shorten the time. The development cycle of new drugs. [0003] For example, patent CN207992061U discloses a high-throughput drug screening imaging device, which is based on fluorescence imaging means, and can simultaneously realize the reaction and detection of multiple samples by using a multi-well plate as a culture chamber, thereby enabling high-throughput drug screening. However, if the device is used to carry out drug screening experiments with multiple concentratio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34C12M1/00B01L3/00
CPCC12M23/16C12M41/46C12M29/00B01L3/5027B01L2200/10
Inventor 刘松柏常宏
Owner SUZHOU HEALTH COLLEGE