Unlock instant, AI-driven research and patent intelligence for your innovation.

Micro-extraction method of acaricide in curcuma wenyujin

An extraction method and acaricide technology, which are applied in the field of micro-extraction of acaricides in Wenyujin, can solve the problems of long extraction time, short extraction time, large amount of solvent, etc., and achieve short extraction time, less impurity content, and improved precision. degree of effect

Active Publication Date: 2021-12-03
HANGZHOU NORMAL UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems of large amount of solvent, long extraction time and complicated operation in the method for extracting residual acaricides in Chinese herbal medicines in the prior art, the present invention provides a micro-extraction method of acaricides in Curcuma melliferae. The extraction operation of the invention is simple, The amount of organic solution is less, the extraction time is short, and the enrichment effect is good

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro-extraction method of acaricide in curcuma wenyujin
  • Micro-extraction method of acaricide in curcuma wenyujin
  • Micro-extraction method of acaricide in curcuma wenyujin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A. Dissolve 1g of turmeric powder in 15mL of acetonitrile, add 2.0g of NaCl and 4.0g of MgSO 4 , use an ultrasonic instrument to sonicate for 30 minutes and then centrifuge, add the supernatant to a C18 adsorbent solid-phase extraction column prewashed with acetonitrile for mixed adsorption, and collect the solution containing unadsorbed components;

[0048] B. Take the solution containing unadsorbed components and mix it with cucurbituril aqueous solution, adjust the pH to 7.0, and dilute to 10.00mL. After dilution, the concentration of cucurbituril is 0.2μg / mL, and the concentration of each component acaricide is about 0.2μg / mL , shake the solution at a speed of 500rpm for 60s, and then filter the mixed solution with a 0.45μm disposable nylon filter;

[0049] C. Elute the residual particles on the filter membrane with 100 μL of methanol, centrifuge the solution obtained by the elution, take the supernatant and filter it with a 0.22 μm nylon filter, and take the filtra...

Embodiment 2

[0053] A. Dissolve 1g of turmeric powder in 15mL of acetonitrile, add 2.0g of NaCl and 4.0g of MgSO 4 , use an ultrasonic instrument to sonicate for 30 minutes and then centrifuge, add the supernatant to a C18 adsorbent solid-phase extraction column prewashed with acetonitrile for mixed adsorption, and collect the solution containing unadsorbed components;

[0054] B. Take the solution containing unadsorbed components and mix it with the cucurbituril aqueous solution, adjust the pH to 7.0, and dilute to 10.00mL. After dilution, the concentration of cucurbituril is 0.2μg / mL, and the concentration of each component acaricide is about 2μg / mL. Shake the solution at a speed of 500rpm for 60s, then filter the mixed solution with a 0.45μm disposable nylon filter;

[0055] C. Elute the residual particles on the filter membrane with 100 μL of methanol, centrifuge the eluted solution, take the supernatant and filter it with a 0.22 μm nylon filter, and take the filtrate for detection and...

Embodiment 3

[0057] A. Dissolve 1g of turmeric powder in 15mL of acetonitrile, add 2.0g of NaCl and 4.0g of MgSO 4 , use an ultrasonic instrument to sonicate for 30 minutes and then centrifuge, add the supernatant to a C18 adsorbent solid-phase extraction column prewashed with acetonitrile for mixed adsorption, and collect the solution containing unadsorbed components;

[0058] B. Mix the solution containing unadsorbed components with the cucurbituril aqueous solution, adjust the pH to 7.0, and dilute to 10.00mL. After dilution, the concentration of cucurbituril is 0.4 μg / mL, and the concentration of each component of the acaricide is about 2 μg / mL. Shake the solution at a speed of 500rpm for 60s, then filter the mixed solution with a 0.45μm disposable nylon filter;

[0059] C. Elute the residual particles on the filter membrane with 100 μL of methanol, centrifuge the eluted solution, take the supernatant and filter it with a 0.22 μm nylon filter, and take the filtrate for detection and an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of extraction and enrichment of acaricide pesticide residue analytes, aims to solve the problems of large solvent dosage, long extraction time and complex operation of a method for extracting a residual acaricide in Chinese herbal medicines in the prior art, and discloses a micro-extraction method of an acaricide in curcuma wenyujin, which comprises the following steps: dissolving curcuma wenyujin powder in a solvent, centrifuging after ultrasonic treatment, adding supernate into a solid-phase extraction column for mixed adsorption, and collecting a solution containing unadsorbed components; mixing the solution containing unadsorbed components with a cucurbituril aqueous solution, adjusting the pH value, shaking, and filtering the mixed solution by using a filter membrane; and eluting the residual particles on the filter membrane by using an eluent, wherein the eluted solution contains the extracted acaricide. The extraction operation is simple, the extraction time is short, the enrichment efficiency is high, the impurity content after extraction and enrichment is low, and the use amount of an organic solution used in the whole extraction process is small.

Description

technical field [0001] The invention relates to the field of extraction and enrichment of acaricide-like pesticide residue analytes, in particular to a micro-extraction method of acaricide in curcuma. Background technique [0002] In the cultivation of fruit trees and herbs, acaricides are commonly used to control the growth of insects and mites to minimize the adverse effects of diseases and pests on crops. However, the widespread use of acaricides has caused environmental and food pollution, and acaricide residues in food and Chinese herbal medicines will damage public health. Therefore, it is necessary to establish a sensitive, efficient and suitable analytical method to detect the content of acaricides in Chinese herbal medicines. The commonly used method for detecting acaricides is instrumental analysis. Due to the complex components in Chinese herbal medicine samples and the low content of acaricides, it is necessary to extract and enrich the acaricides in Chinese her...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/08
CPCG01N30/06G01N30/08G01N2030/062G01N2030/027
Inventor 曹君顾郁欣乐紫璇闫天赐
Owner HANGZHOU NORMAL UNIVERSITY