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Fluorescent probe for detecting CYP450 as well as preparation method and application of fluorescent probe

A fluorescent probe and reaction solution technology, applied in the field of chemistry, to achieve the effect of reducing interference and high detection accuracy

Active Publication Date: 2021-12-10
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, neither of the above two fluorescent probes has been used to directly study CYP450

Method used

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  • Fluorescent probe for detecting CYP450 as well as preparation method and application of fluorescent probe
  • Fluorescent probe for detecting CYP450 as well as preparation method and application of fluorescent probe
  • Fluorescent probe for detecting CYP450 as well as preparation method and application of fluorescent probe

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Embodiment Construction

[0029] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0030] The first part, the structure and preparation method of the fluorescent probe for detecting CYP450 1, the structure of the fluorescent probe

[0031] The fluorescent probe for detecting CYP450 provided by the present invention is an organic compound based on N-oxide linking tetraphenylethylene compound (TPE), which is recorded as fluorescent probe TPE-CYP, and its structural formula is as shown in formula I:

[0032]

[0033] 2. Preparation method of fluorescent probe TPE-CYP

[0034] refer to image 3 , the preparation method of fluorescent probe TPE-CYP shown in formula I specifically comprises the following steps:

[0035] (1) Compound 1 was synthesized: benzophenone (18.2g, 2mmol), 4-hydroxybenzophenone (9.9g, 1mmol) and zinc powder (13g, 4mmol) were placed under anhydrous THF (300mL) and nitrogen protection Reflux, slowl...

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Abstract

The invention discloses an aggregation-induced emission fluorescent probe for detecting CYP450 and a preparation method and application thereof. The fluorescent probe is an organic compound based on N-oxide connected tetraphenyl ethylene compounds, and the structural formula of the fluorescent probe is shown in the invention; the fluorescence intensity of the fluorescent probe TPE-CYP changes in the oxygen-deficient environment and in the presence of CYP450, the probe can be used for qualitative and quantitative detection of CYP450, can greatly reduce interference of external detection conditions, and has high detection precision.

Description

technical field [0001] The invention relates to a fluorescent probe and its preparation method and application, in particular to an aggregation-induced emission fluorescent probe for detecting CYP450 (cytochrome P450 enzyme) and its preparation method and application, belonging to the field of chemical technology. Background technique [0002] Cancer is a complex disease with high annual morbidity and mortality. However, cancer drug resistance greatly reduces the cure rate of tumors, and cytochrome P450 enzymes (CYP450) play an important role in the formation of tumor cisplatin resistance. In clinical practice, more than 90% of drugs metabolized by the liver involve CYP450 subtypes. Therefore, a better understanding of the relationship between CYP450 and cancer drug resistance will improve existing therapeutic strategies and facilitate the development of personalized treatments. Small molecule fluorescent probes have a variety of chemical structure modifications, which mak...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D239/91C09K11/06G01N21/64
CPCC07D239/91C09K11/06G01N21/6428C09K2211/1007C09K2211/1044Y02P20/55
Inventor 王晓艳黄严鹿鹏鹏付丽丽孙明召王凌霄陈令新
Owner BINZHOU MEDICAL COLLEGE
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