KASP molecular marker for peanut pod and kernel size major QTL and application

Abstract

The invention relates to the technical field of molecular genetic breeding, and discloses a KASP molecular marker of peanut pod and kernel size major QTL. The KASP molecular marker comprises SNP1 and SNP2; the molecular marker primers SNP1 include a forward primer I, a forward primer II and a universal reverse primer I; and the molecular marker primers SNP2 include a forward primer III, a forward primer IV and a universal reverse primer II. The invention further discloses application. The KASP molecular marker disclosed by the invention is proper in genetic distance, the positioned peanut pod and kernel size major QTL is high in heritability, the technology is reliable, economical, simple and convenient, high-throughput typing can be realized, two important yield traits of peanut underground pod and kernel sizes can be simultaneously subjected to marker-assisted selection, the identification is accurate and rapid, the complex process of peanut underground pod breeding can be effectively avoided, the breeding efficiency is improved, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of molecular genetic breeding, in particular to the KASP molecular marker and application of peanut pod and kernel size main effect QTL. Background technique [0002] Peanut is one of the main oil crops and economic crops in my country. The planting area is maintained at 70 million mu all year round, with a total annual output of 17 million tons. Peanut oil and peanut products occupy an important position in the development of my country's agricultural economy. Industrial development is of great significance. However, due to the limited arable land area, there is not enough space to increase the total yield by increasing the sown area. Therefore, under the current stable sown area, increasing the per unit yield is the most effective way to achieve stable and high peanut production in my country. Studies have shown that peanut pod size has the most direct contribution to yield, but because peanut is a crop th...

AI Technical Summary

Problems solved by technology

[0004] At present, the development of SNP markers based on KASP technology and the application of assisted breeding for peanut yield traits have not been reported. Therefore, in order to effectively avoid the complicated process of underground pod breeding of peanuts and improve the efficiency of peanut breeding, the inventors carried out research on the traits of peanut pods and kernels. QTL mapping, and the development of SNP molecular markers based on sequence information, and the establishment of early assisted selection methods for peanut yield traits in hybrid progeny

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