Kasp molecular marker and application of major qtl for peanut pod and kernel size

Abstract

The invention relates to the technical field of molecular genetics and breeding, and discloses KASP molecular markers of major QTLs for peanut pod and seed kernel size, including SNP1 and SNP2, and the SNP1 molecular marker primers include forward primer 1, forward primer 2 and universal reverse Primer 1, the SNP2 molecular marker primers include forward primer 3, forward primer 4 and universal reverse primer 2; applications are also disclosed. The KASP molecular marker of the invention has suitable genetic distance, high heritability of the main effect QTL for the size of peanut pods and seed kernels, reliable, economical and simple technology, can realize high-throughput typing, and can simultaneously determine the size of underground pods and seed kernels of peanuts. Marker-assisted selection of two important yield traits is accurate and rapid in identification, which can effectively avoid the complicated process of peanut underground pod breeding, improve the breeding efficiency, and has broad application prospects.

Description

technical field

[0001] The invention relates to the technical field of molecular genetic breeding, in particular to the KASP molecular marker and application of the main QTL for peanut pod and seed kernel size. Background technique

[0002] Peanut is one of the main oil crops and economic crops in my country. The planting area is maintained at 70 million mu all year round, and the total annual output is 17 million tons. Peanut oil and peanut products play an important role in the development of my country's agricultural economy. Industrial development is of great significance. However, due to the limited area of ​​arable land, there is not enough space to increase the total yield by increasing the sown area. Therefore, under the current stable sown area, increasing the unit yield is the most effective way to achieve stable and high yield of peanuts in my country. Studies have shown that peanut pod size has the most direct contribution to yield, but because peanut is a "above...

Examples

Embodiment

[0018] In this example, 10X whole-genome resequencing was performed on 390 peanut resource varieties from 15 countries around the world, and a total of 12.93Tb sequencing data was obtained. After quality control filtering, 2,568,601 SNPs were finally screened to construct a high-density genetic variation map. Then, use the genetic variation map to carry out population structure analysis to clarify the different subgroup structures of the associated groups, and obtain the Q matrix of the population structure; at the same time, carry out the evaluation of the relationship between individuals in the associated group to obtain the K matrix of the relationship. Eight main yield traits including pod length, pod width, pod thickness, 100 pod weight, seed kernel length, seed kernel width, seed kernel thickness and 100 kernel weight under four different environments were investigated. In order to reduce the impact of environmental effects on the results of association analysis, the bes...

technical field

[0001] The invention relates to the technical field of molecular genetic breeding, in particular to the KASP molecular marker and application of the main QTL for peanut pod and seed kernel size. Background technique

[0002] Peanut is one of the main oil crops and economic crops in my country. The planting area is maintained at 70 million mu all year round, and the total annual output is 17 million tons. Peanut oil and peanut products play an important role in the development of my country's agricultural economy. Industrial development is of great significance. However, due to the limited area of ​​arable land, there is not enough space to increase the total yield by increasing the sown area. Therefore, under the current stable sown area, increasing the unit yield is the most effective way to achieve stable and high yield of peanuts in my country. Studies have shown that peanut pod size has the most direct contribution to yield, but because peanut is a "above...

Examples

Embodiment

[0018] In this example, 10X whole-genome resequencing was performed on 390 peanut resource varieties from 15 countries around the world, and a total of 12.93Tb sequencing data was obtained. After quality control filtering, 2,568,601 SNPs were finally screened to construct a high-density genetic variation map. Then, use the genetic variation map to carry out population structure analysis to clarify the different subgroup structures of the associated groups, and obtain the Q matrix of the population structure; at the same time, carry out the evaluation of the relationship between individuals in the associated group to obtain the K matrix of the relationship. Eight main yield traits including pod length, pod width, pod thickness, 100 pod weight, seed kernel length, seed kernel width, seed kernel thickness and 100 kernel weight under four different environments were investigated. In order to reduce the impact of environmental effects on the results of association analysis, the bes...

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