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Method for improving capture of circulating tumor cells in blood

A tumor cell and blood technology, applied in cell dissociation methods, tumor/cancer cells, animal cells, etc., can solve the problem of inaccurate CTC numbers and achieve the effect of improving efficiency and accuracy

Pending Publication Date: 2021-12-14
安徽贝铭生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the inaccurate defect of the number of CTCs detected in the blood in the prior art, and to provide a method that utilizes the specific expression of ofCS (carcinoembryonic chondroitin sulfate) on the surface of almost all tumor cells and VAR2 The specificity of combining with ofCS, using rVAR2 to capture CTCs fluorescently, improving the number and sensitivity of CTC detection

Method used

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  • Method for improving capture of circulating tumor cells in blood
  • Method for improving capture of circulating tumor cells in blood
  • Method for improving capture of circulating tumor cells in blood

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Embodiment

[0028] A method for improving the capture of tumor cells in blood circulation, comprising the steps of:

[0029] S1. Filter the blood to be tested through the membrane chip, such as: FR1 detection system, leaving CTCs on the membrane; The FR1 detection system (Wanhe Equipment No. 20200336) is an ultra-low pressure system that can capture tumor cells in human blood with high efficiency and high purity, and is used for early diagnosis of tumors and precise treatment of tumors. Blood passes through the membrane chip under low pressure conditions. Because tumor cells are larger than normal white blood cells, they cannot pass through the membrane pores and remain on the membrane. The cells remaining on the membrane are mainly white blood cells and a small amount of tumor cells. Red blood cells are almost completely filtered out due to their reduced volume. Gently wash the membrane, remove some red blood cells, and make the surface of the membrane relatively clean;

[0030] S...

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PUM

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Abstract

The invention discloses a method for improving capture of circulating tumor cells in blood, which comprises the following steps: S1, filtering blood to be detected through a membrane chip, and leaving CTC on a membrane; S2, adding a fluorescence labeled rVAR2 / anti-CD45 fluorescent antibody / DAPI (4, 4'-diaminodiphenylmethane) onto the membrane; and S3, performing CTC counting through a fluorescence microscope. The CTC captured by rVAR2 fluorescent staining is used, the capture / recognition of the CTC does not depend on the expression of a single protein on the surface of the CTC, and the CTC derived from epithelial cells and the CTC with interstitial cell properties are captured together as much as possible; and therefore, the capturing efficiency and accuracy are improved, and the capturing method is economical, simple and convenient.

Description

technical field [0001] The present invention relates to a method for improving the capture of tumor cells in blood circulation. Background technique [0002] CTC refers to tumor cells that enter the blood circulation. The CTC test is a test to detect floating cancer cells in the blood. Ultra-early micro-carcinomas that cannot be detected by imaging examinations can be identified through CTC examinations. Clinically, tumors can be detected earlier than traditional diagnostic methods, and the incidence of ultra-early primary cancers, as well as ultra-early cancer metastasis or relapse. CTC in patients with lung cancer, rectal cancer, breast cancer, prostate cancer, pancreatic cancer and other tumors is an important biomarker in the blood to observe the prognosis and treatment effect of tumors. The more the number of CTCs in the blood of tumor patients, the worse the prognosis of the patients. [0003] At present, there are two main categories of CTC capture techniques base...

Claims

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Application Information

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IPC IPC(8): C12N5/09
CPCC12N5/0694C12N2509/00
Inventor 鲍伟胜鲍岳洋王春雷
Owner 安徽贝铭生物科技有限公司
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