Tracer composition
A composition and tracer technology, which can be used in biocides, animal repellents, plant growth regulators, etc., can solve expensive, destroyed, time-consuming and other problems
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example 1
[0147] Example 1 - Determining Suitable Fluorescent Tracers
[0148] First, the inventors wanted to identify a fluorescent tracer that could be used in a method of detecting and analyzing the extent and thoroughness of spray deposition. Ideally, a suitable tracer should:
[0149] a) have a high fluorescence quantum yield;
[0150] b) It can be detected under the condition that the background fluorescence interference of plants is minimal;
[0151] c) soluble in water;
[0152] d) No need to use pH buffer;
[0153] e) have sufficient photostability properties;
[0154] f) is safe and acceptable for use on food crops; and
[0155] g) cost-effective.
[0156] Initially, 140 potential compounds were identified. Considering the above factors, the initial screen left 21 possible compounds. The Excitation and Emission Spectroscopy Matrix (EEM) of the compounds in solution and solid phase was measured using CTG's Horiba Aqualog, and the solution results for four of the poten...
example 2
[0164] Example 2——improving the fluorescence properties of FMN-Na
[0165] like image 3 and 4 As shown, FMN-Na exhibits low quantum yield and very poor photostability when dry.
[0166] like Figure 5 As shown, the inventors found that the fluorescent properties of FMN-Na could be improved by combining FMN-Na with a gelling agent. Specifically, sucrose and glycerin were used because these compounds are safe and edible. Remarkably, both compounds improved the fluorescence properties of FMN-Na when used alone, but provided exceptionally significant improvements when used in combination.
example 3
[0167] Example 3——improving the stability of FMN-Na
[0168] The inventors found that FMN-Na was unstable in sunlight, and neither sucrose nor glycerol improved its stability. Therefore, the inventors tested the effects of various compounds on the photodegradation of FMN-Na, and the results were in Image 6 shown in. The two most effective compounds tested were ascorbic acid and sodium thiosulfate.
[0169] Figure 7 UV absorbance spectra of FMN-Na, ascorbic acid and sodium thiosulfate are shown. Notably, ascorbic acid closely matches the absorbance spectrum of riboflavin between 220 and 300 nm. Without wishing to be bound by theory, it is believed that by absorbing light between these wavelengths, ascorbic acid prevents photodegradation of FMN-Na. Notably, ascorbic acid is also a free radical scavenger. Therefore, this effect may also be responsible for the improved stability of FMN-Na.
[0170] To investigate this, the inventors prepared a tracer composition compris...
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