Construction method of clinical-grade human umbilical cord mesenchymal stem cell resource library multi-stage library
A construction method and technology of mesenchymal stem cells, which is applied in the field of multi-level library construction of clinical-grade human umbilical cord mesenchymal stem cell resource bank, can solve the problems of no standardized method, preparation technology and detection technology can not be unified, to achieve quality controllable, The effect of high cell viability
Pending Publication Date: 2021-12-24
SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
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Problems solved by technology
[0004] At present, most of the preservation of umbilical cord stem cells in domestic stem cell banks is based on scientific research, and the preparation technology and detection technology used cannot be unified, and there is no standardized method
Method used
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Embodiment 1
[0059] Seed bank establishment plan:
[0060] Donor Screening: Donor testing items: lymphocytic leukemia virus antibody detection, hepatitis C virus antibody, cytomegalovirus IgM antibody, hepatitis B virus surface antigen, human immunodeficiency virus antibody and antigen, Treponema pallidum antibody, hepatitis B Viral nucleic acid, hepatitis C virus nucleic acid, human immunodeficiency virus nucleic acid, and EBV virus IgM antibody tests were negative, healthy women with no family history of infectious diseases, no history of drug abuse, and aged between 20 and 35.
[0061] Raw material collection: cut the umbilical cord 5-10 cm, clamp both ends of the umbilical cord with disposable hemostatic clips, put the umbilical cord into a medical ziplock bag, paste the donor barcode, put it into a 4°C transfer box, infectious disease test report, informed consent and The collection record form is packed into a material bag and transported to the preparation room.
[0062] Raw materi...
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The invention relates to a construction method of a clinical-grade human umbilical cord mesenchymal stem cell resource library multi-stage library, and belongs to the technical field of cell culture. According to the construction method, raw material pretreatment, stem cell separation and multiplication culture are performed to obtain a seed bank, and then construction of a main bank, a working bank and a product bank is realized by setting conditions such as inoculation density and culture time. According to the construction method, the cell viability is high, the quality is controllable, and clinical level four-level stem cell resource libraries, namely the seed bank, the main bank, the working bank and the product bank, can be successfully constructed.
Description
technical field [0001] The invention relates to the technical field of cell culture, in particular to a method for constructing a multi-level library of a clinical-grade human umbilical cord mesenchymal stem cell resource bank. Background technique [0002] As the most populous country in the world, and also as a developing country that is entering an aging society, my country is facing many challenges in terms of population health. These challenges are reflected in the fact that the high incidence is gradually changing from infectious diseases to chronic diseases and senile degenerative diseases, the incidence of various tumors is becoming younger, and the incidence of tissue and organ dysfunction caused by various factors such as genetic trauma and metabolism continuously rising. For these diseases, traditional drug therapy and surgical treatment are mostly ineffective, and cell-, tissue- or organ-based replacement therapy is expected to be the best option to cure these d...
Claims
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Patent Timeline
Login to View More IPC IPC(8): C40B50/06C40B40/02C12N5/0775A01N1/02
CPCC40B50/06C40B40/02C12N5/0665A01N1/0221A01N1/0284C12N2509/00C12N2509/10C12N2513/00
Inventor 刘中民赵庆辉汤红明许啸
Owner SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE