Use of Gastrodia elata polypeptide in the preparation of external protective products against new coronavirus
A gastrodiae and virus technology, applied in the field of medicine, can solve the shortage of anti-new coronavirus external products, achieve high commercial development value, not easy to residue, and protect people's lives and safety
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Embodiment 1
[0029] 1) Gastrodia elata is washed and removed from impurities, crushed to 80 mesh, placed in 0.6% sodium chloride solution at a solid-liquid ratio of 1:6 (g / mL), and extracted in a water bath at 80°C for 4 hours;
[0030] 2) After filtration under reduced pressure, the obtained Gastrodia elata protein extract is concentrated to a concentration of the original drug of 1 (g / mL);
[0031] 3) Add 0.4% compound enzyme hydrolyzate by volume ratio to the concentrate, containing 1.5% papain, 0.8% trypsin and 0.6% flavor protease in the compound enzyme hydrolyzate, the enzymolysis temperature is 55°C, and the enzymolysis time is After 40 minutes, pass the enzymatic solution through a 10KDa membrane for ultrafiltration, and centrifuge at 8000rpm for 20 minutes. The filtrate obtained under the membrane is the Gastrodia elata polypeptide extract, which is stored at 4°C for future use.
[0032] 4) SDS-PAGE electrophoresis method was used to measure the molecular weight of Gastrodia elata...
Embodiment 2
[0034] 1) Gastrodia elata is washed and removed of impurities, crushed to 100 mesh, placed in a 0.4% sodium chloride solution at a solid-liquid ratio of 1:8 (g / mL), and extracted in a water bath at 80°C for 2 hours;
[0035] 2) After vacuum filtration, the obtained Gastrodia elata protein extract is concentrated to a concentration of the original drug of 0.8g / mL;
[0036] 3) Add 0.2% compound enzyme hydrolyzate by volume ratio to the concentrate, containing 2% papain, 0.6% trypsin, and 0.5% flavor protease in the compound enzyme hydrolyzate, the enzymolysis temperature is 50°C, and the enzymolysis time is After 30 minutes, pass the enzymatic solution through a 10KDa membrane for ultrafiltration, and centrifuge at 8000rpm for 20 minutes. The obtained filtrate is the Gastrodia elata polypeptide extract, which is stored at 4°C for later use;
[0037] 4) SDS-PAGE electrophoresis method was used to measure the molecular weight of Gastrodia elata polypeptide, and the ultra-low molec...
Embodiment 3
[0039]1) Gastrodia elata is washed and removed from impurities, crushed to 40 mesh, placed in a 1% sodium chloride solution at a solid-to-liquid ratio of 1:12 (g / mL), and extracted in a water bath at 80°C for 6 hours;
[0040] 2) After filtration under reduced pressure, the obtained Gastrodia elata protein extract is concentrated to a concentration of the original drug of 0.5 g / mL;
[0041] 3) Add 0.8% compound enzyme hydrolyzate by volume to the concentrate, containing 3% papain, 1.5% trypsin and 1% flavor protease in the compound enzyme hydrolyzate, the enzymolysis temperature is 60°C, and the enzymolysis time is 1.5h, then pass the enzymatic solution through a 10KDa membrane for ultrafiltration, centrifuge at 8000rpm for 20min, the obtained filtrate is the Gastrodia elata polypeptide extract, and store it at 4°C for later use;
[0042] 4) SDS-PAGE electrophoresis method was used to measure the molecular weight of Gastrodia elata polypeptide, and the ultra-low molecular weig...
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