Treatment method for relieving dormancy of terminal buds of peony tissue culture seedlings

A treatment method and a technique for tissue cultured seedlings, which are applied in the field of treatment for relieving dormancy of top buds of peony tissue cultured seedlings, and can solve the problems of low survival rate of transplanting

Active Publication Date: 2022-01-04
河南省农业科学院园艺研究所
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AI Technical Summary

Problems solved by technology

But overall, the technical problem of the low survival rate of transplanting is still relatively prominent

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0040] Taking a specific peony variety as an example, the treatment method for releasing the dormancy of the top buds of peony tissue culture seedlings provided by this application is specifically introduced as follows.

[0041] (1) Harvest explants and induce germination

[0042] Collect peony scale buds from Henan area (Luoyang) in February as explants, after disinfection and sterilization, transfer them to bud induction medium to induce germination to form seedlings;

[0043] In this example, the peony variety "Daojin" that is 5-10 years old and grows vigorously is selected, and the mother plant without diseases and insect pests is selected, and its scale buds (full-growing axillary buds in the middle and lower parts of the branches) are collected as the source of explants.

[0044] When disinfecting and sterilizing the scale buds, refer to the following operations for details:

[0045] Rinse the collected scale buds with tap water for 30 minutes and take them to the ultra...

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Abstract

The invention belongs to the technical field of tissue culture, and particularly relates to a treatment method for relieving dormancy of terminal buds of peony tissue culture seedlings. The method comprises the following steps of collecting explants, inducing germination, carrying out multiplication culture, inducing rooting, inducing terminal buds, breaking dormancy and the like. The inventor believes that the main reasons of low transplanting survival rate of existing peony tissue culture seedlings are as follows: firstly, after induced roots of the tissue culture seedlings grow, terminal bud dormancy phenomenon exists, and growth of overground parts is stopped; and secondly, although the rooting rate effect of the existing peony tissue culture seedling is improved to a certain extent, a root system is not developed, and along with the dormancy of the terminal buds and the reduction of nutritional requirements, the growth vitality of the root system after rooting is further weakened, and the survival rate after transplanting is lower. Aiming at the problems, on one hand, the inventor improves the rooting induction effect by optimizing related culture media and culture conditions, on the other hand, terminal bud dormancy is relieved and the growth vigor is improved by regulating and controlling the environment, so that root system germination and growth are promoted, and the transplanting survival rate is finally improved.

Description

technical field [0001] The application belongs to the technical field of tissue culture, and in particular relates to a treatment method for relieving dormancy of terminal buds of tree peony tissue culture seedlings. Background technique [0002] Peony, a woody flower of Paeoniaceae Paeoniaceae, has high ornamental, oil and edible value, and is a famous economic crop in my country. [0003] The traditional propagation methods of peony are mainly seeding, branching and grafting, etc., but these traditional propagation methods have obvious defects such as low reproductive efficiency and unstable varieties. Therefore, from the perspective of rapid propagation and stable propagation, the use of tissue culture technology for seedling propagation is currently the most feasible way of peony propagation. However, in actual production, the application of peony tissue culture technology is relatively limited, the main reason is that the rooting effect of tissue culture seedlings is p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/06A01G31/00A01G22/20
CPCA01H4/008A01H4/002A01G7/06A01G31/00A01G22/20Y02A40/22Y02P60/40
Inventor 符真珠张和臣李艳敏王慧娟王二强王利民高凯高杰袁欣卢林
Owner 河南省农业科学院园艺研究所
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