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Porous material precursor composition, porous material and preparation method

A technology of porous materials and precursors, which is applied in the field of porous materials and their preparation, and precursor compositions of porous materials, can solve the problems of difficult operation, limited pore-forming effect, long time consumption, etc., and achieves broad application prospects and good biocompatibility. sexual effect

Pending Publication Date: 2022-01-07
苏州永沁泉智能设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This is difficult and time-consuming in terms of product application, which is not conducive to commercial transformation
In addition, the patent uses a single emulsion phase separation pore-forming mechanism, and its pore-forming effect is limited

Method used

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  • Porous material precursor composition, porous material and preparation method
  • Porous material precursor composition, porous material and preparation method
  • Porous material precursor composition, porous material and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Hydrogel precursor polymer, porogen, photoinitiator, photofoaming agent are dropped into 10mL deionized water according to the material and material amount of each group in Example 1 in Table 1 (the weight and volume percentage in Table 1 is based on Calculated based on the volume of deionized water), heat and stir at 50°C in the dark to dissolve; freeze-dry the above hydrogel precursor solution; heat and stir the freeze-dried hydrogel precursor in 10mL PBS at 37°C in the dark , dissolved into a hydrogel precursor solution, filtered and sterilized, and digested and centrifuged MC3T3-E1 cells (in terms of the volume of the gel obtained, the final planting density was 5*10 6 / mL) were mixed evenly to prepare a cell-containing hydrogel precursor solution, which was injected into a culture vessel and irradiated with a 405nm light source to obtain scaffolds 1-5 (obtained according to the material ratio of 1-5 groups) and scaffolds 1- 18+5 (obtained according to the material ...

Embodiment 2

[0061] According to the materials and material ratios of each group in Example 2 in Table 1, referring to the steps in Example 1, respectively prepare support 2-5, support 2-14+5, support 2-14+5+3;

[0062] Cultivate according to the method of Example 1, and use an optical inverted microscope to take pictures on the 1st and 4th days;

[0063] Such as figure 2 As shown, the scaffolds obtained by the 2-14+5 group and the 2-14+5+3 group showed obvious stretching on the 1st day, while the stretching of the scaffold 2-5 (only adding 5wt% GelMA and photoinitiator) was less; On the 4th day, all the three groups proliferated and stretched, and the 2-14+5+3 scaffold obtained in the 2-14+5+3 group was the most obvious, because the porous structure promotes nutrient transmission and waste discharge, so compared with the untreated Cells proliferate and expand in the porous GelMA (scaffold 2-14+5+3) prepared by phase separation combined with foaming method when adding porogen and photofo...

Embodiment 3

[0065] According to the materials and material ratios of each group in Example 3 in Table 1, referring to the steps in Example 1, respectively prepare support 3-5, support 3-8+5+3, support 3-14+5+3;

[0066] According to the culture method of Example 1, CCK-8 detection was performed on the scaffolds cultivated to 1, 4, and 7 days, and the absorbance of the CCK-8 working solution was measured with a microplate reader to quantitatively analyze the proliferation of cells on the scaffolds. For the proliferation of cells on the 4th and 7th day, see image 3 .

[0067] Depend on image 3 It can be seen that on the 4th day of culture, the 5wt% group (that is, the scaffolds 3-5 obtained from the 3-5 group) proliferated slightly, and the other two groups all proliferated more obviously. When cultured to the 7th day, the scaffold 3-8+5+3 obtained by the 3-8+5+3 group was higher than that of the blank hydrogel group (ie, the 3-5 group), and the 3-14+5+3 group The obtained scaffold 3-1...

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Abstract

The invention discloses a porous material precursor composition which comprises a precursor polymer, a pore-foaming agent, a photoinitiator and a photofoaming agent. The invention also discloses a method for preparing a porous material by using the composition and the porous material. Microscopic pore channels in a hydrogel provide channels for exchange of cells and external substances and provide space for proliferation and growth of the cells. Compared with the traditional non-porous hydrogel, the porous hydrogel provided by the invention can effectively improve the cell proliferation activity. The porous hydrogel disclosed by the invention is simple to prepare, can be formed in situ, and has a wide application prospect in the fields of cell culture and tissue engineering.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a porous material precursor composition, a porous material and a preparation method. Background technique [0002] Hydrogel is a water-rich three-dimensional polymer network, which has been widely used in the field of tissue engineering due to its high hydrophilicity and structural similarity to extracellular matrix. [0003] Although hydrogel can simulate the three-dimensional structure of extracellular matrix, its too tight polymer network will limit the cells to absorb nutrients from the outside world and discharge metabolic waste. In order to enhance the material exchange between the cells in the hydrogel and the outside world, it is usually necessary to increase the microscopic pore size of the gel material. The current common solutions include using gelatin particles as sacrificial materials to cause pores, emulsion mixing, 3D printing channel structures, etc. These so...

Claims

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Application Information

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IPC IPC(8): C08J9/10C08J9/26C08L89/00C08L5/08C12N5/00C12N5/071C12N5/077C12N5/0775A61L27/52A61L27/56A61L27/20A61L27/22A61L27/38
CPCC08J9/102C08J9/26C12N5/0062C12N5/0663C12N5/0691C12N5/0654A61L27/52A61L27/56A61L27/20A61L27/222A61L27/3895C12N2513/00C12N2533/54C12N2533/80C12N2533/72C08J2203/04C08J2389/00C08J2305/08C08J2201/0464C08J2201/0482C08J2205/022C08J2207/10C08L5/08
Inventor 高庆王鹏简宇航李美琪
Owner 苏州永沁泉智能设备有限公司
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