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SNP molecular marker for dairy cow milk heat resistance screening and application in breeding field

A molecular marker and dairy cow technology, applied in the field of dairy cow genetic improvement, to reduce breeding costs and invisible economic losses, and shorten the breeding time

Pending Publication Date: 2022-01-07
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GRM8 gene may be a candidate gene affecting milk fever resistance in dairy cows, and its genetic variation (SNP mutation) is the potential genetic basis for the differences in milk fever resistance among different dairy cows, but GRM8 mutations are closely related to milk fever in dairy cows. Studies on the relationship have not been reported

Method used

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  • SNP molecular marker for dairy cow milk heat resistance screening and application in breeding field
  • SNP molecular marker for dairy cow milk heat resistance screening and application in breeding field
  • SNP molecular marker for dairy cow milk heat resistance screening and application in breeding field

Examples

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Embodiment 1

[0041] In this example, the identification of molecular markers related to the resistance to lactation heat of dairy cows and its application methods according to the present invention are described in detail.

[0042] 1. Screening the GRM8 gene as a candidate gene for milk fever resistance in dairy cows

[0043] (1) Collect blood samples from 2709 individual Holstein cattle from 11 pastures in various regions of my country. The location of the 11 pastures in each region and the number of blood samples collected from each pasture are shown in Table 2. Genomic DNA was extracted using conventional methods. The OD260 / 280 of the DNA sample should be between 1.7-2.1. The SNP chip experiment of each sample requires more than 500ng of genome DNA.

[0044] Table 1 Location of pastures where samples were collected and number of samples collected

[0045]

[0046] (2) The genotypes of the collected samples were determined using the Illumina BovineSNP50K chip, and a total of 47,843 ...

Embodiment 2

[0064] Select 294,268,399 dairy cows from the core population of Chinese Holstein cattle in three different regions, extract the DNA in the blood of the cows, use the above primers for PCR amplification, and then send it to a sequencing company for sequencing, and use DANMAN and SeqMan for sequence analysis The software analyzes the sequencing results to obtain the genotype and allele frequency of the BTA4:g.92099005G>A site on the bovine chromosome 4. The detection results are shown in Table 3. The detection results show that there are three allele types at the BTA4:g.92099005G>A locus in the Chinese Holstein cattle population; in the detected dairy cattle population, the GG genotype frequency is 27.9%, and the GA genotype combination frequency is 51.4% %, AA genotype combination frequency was 20.7%. The GG genotype is the dominant genotype, and individuals with the GG genotype can be selected for genetic improvement, so as to breed new milk cow lines resistant to milk fever....

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Abstract

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker for dairy cow milk heat resistance screening and application in the field of breeding, and belongs to the technical field of dairy cow breeding molecular markers. Milk fever is the most common acute low-calcium metabolic disease in 5-9-year-old dairy cows, may occur in the whole production stage of the dairy cows, is easy to relapse, and has obvious influence on the health condition and milk yield of the dairy cows. Molecular markers related to milk heat resistance of dairy cows are designed and obtained, and female parents with dominant characters are screened in an auxiliary mode for breeding for genetic improvement of the dairy cows. Based on the technical purpose, the invention specifically provides GRM8 gene and the molecular marker influencing the milk heat resistance of the dairy cow, the genotype of the tag SNP of the dairy cow on the gene is identified through sequencing, and individuals with dominant genotypes or haplotypes are selected for genetic improvement, so that the milk heat resistance of the dairy cow group is improved, and the economic loss is reduced.

Description

technical field [0001] The invention belongs to the technical field of dairy cow breeding molecular markers, and specifically relates to a SNP molecular marker related to milk cow milk heat resistance, primers for detecting the SNP molecular marker, a kit and application in the field of dairy cow genetic improvement. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Dairy cows will lose a lot of calcium ions during milk production. Four weeks before calving and four weeks after calving, dairy cows have a sharp increase in calcium demand due to fetal bone development and lactation, and are extremely susceptible to milk fever (Stevenson and Lean, 1998). Milk fever (Milk feve...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 黄金明王鲁豫王金鹏高亚平肖遥魏晓超鞠志花张亚冉杨春红刘文浩王玲玲高运东
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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