A method for establishing an eam mouse model induced by an autoantigen epitope

A technique for establishing a method and a mouse model, which can be applied to instruments for radiological diagnosis, analysis of two-dimensional or three-dimensional molecular structures, image enhancement, etc. Large fluctuations and other problems, to achieve the effect of moderate molding time, low price, and high model stability

Active Publication Date: 2022-03-11
天津医科大学总医院空港医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method can reflect the level of myocardial inflammation and fibrosis to a certain extent, these indicators often fluctuate greatly in the body, which is prone to individual differences, and the stability of indicators is difficult to fully grasp.

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  • A method for establishing an eam mouse model induced by an autoantigen epitope
  • A method for establishing an eam mouse model induced by an autoantigen epitope
  • A method for establishing an eam mouse model induced by an autoantigen epitope

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Embodiment Construction

[0047] Embodiments of the present application will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present application, rather than limiting the scope of the present application. Various objects and advantages of the present application will become apparent to those skilled in the art from the following detailed description of the preferred embodiments.

[0048] In order to further understand the present invention, the embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by...

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Abstract

The present invention provides a method for establishing a mouse model of autoimmune myocarditis (Experimental Autoimmune Myocarditis, EAM) induced by an autoantigen epitope. The relationship between AchR and autoimmune myocarditis is determined for the first time. Injection of AchR-Freund's complete adjuvant solution, AchR-Freund's incomplete adjuvant solution and immune booster to produce antibodies against cardiomyocyte M2 cholinergic receptors to induce autoimmune myocarditis, and the established model has cardiomyocyte hypertrophy , myocardial fibrosis and cardiac dysfunction. At the same time, with 18 F-FDG PET / CT myocardial metabolic imaging is the core, combined with echocardiography, HE staining of myocardial tissue sections, myocardial Mason staining and myocardial tissue immune antibody special staining to verify the characteristic changes related to the mouse model. Provide a new mouse model for further research on autoimmune myocarditis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing an EAM mouse model induced by an autoantigen epitope. Background technique [0002] Myocarditis is an inflammatory disease characterized by myocardial inflammatory cell infiltration and non-ischemic necrosis of myocardial cells. The clinical manifestations of myocarditis vary greatly. Mild cases have no obvious clinical symptoms and are easily overlooked. In severe cases, the myocardium is damaged, leading to arrhythmia, cardiogenic shock or sudden death. The etiology of myocarditis can be divided into infectious factors, such as viruses, bacteria, parasites, chlamydia, fungi, protozoa, etc., and non-infectious factors, such as autoimmune diseases, chemical and drug toxins and allergic reactions. [0003] In the past 2 years, autoimmune myocarditis has received high attention due to the use of PD-1 / PD-L1 monoclonal antibody to treat tumors and t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027G16B15/30G06T7/64G06T7/00A61B6/03A61B6/00G01N33/68
CPCA01K67/027G01N33/6854G16B15/30G06T7/0012G06T7/64A61B6/508A61B6/505A61B6/032A61B6/037A61B6/5217A01K2207/12A01K2227/105A01K2267/0375A01K2207/10A01K2267/0325G06T2207/10081G06T2207/30012
Inventor 张鹏杨照宇王元国陈思宋世辉王欢
Owner 天津医科大学总医院空港医院
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