A kind of establishment method of the eamg mouse model of immunoenhancement

Abstract

The invention provides a method for establishing an immune-enhanced experimental autoimmune myasthenia gravis (Experimental Autoimmune Myasthenia Gravis, EAMG) mouse model. On the basis of AchR peptide segment immune-induced modeling, continuous injection of thymoma The cell line Thy0517 stimulated the cultured dendritic cells, and was supplemented with aluminum hydroxide adjuvant to establish the model. The immune-enhanced EAMG mouse model disclosed in the present invention can make mice by enhancing the antigen presentation ability of dendritic cells. It maintains a stable immune-enhancing state in vivo, and the modeling period is effectively shortened from 110 ± 3 days to 98 ± 3 days. At the same time, the muscle multimodal detection is used to verify the characteristic changes related to the mouse model. For the exploration of myasthenia gravis Research provides a stable model.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for establishing an EAMG mouse model, in particular to a method for establishing an immune-enhanced EAMG mouse model. Background technique [0002] Myasthenia gravis (MG) is an autoimmune disease characterized by the body producing autoantibodies that attack different targets at the neuromuscular junction, causing muscle fatigue and weakness. According to the target of autoantibodies, MG can be divided into muscle-type acetylcholine receptor (AchR)-targeted myasthenia; MG caused by autoantibodies binding to muscle-specific tyrosine kinase; autoantibodies attacking lectin receptors. Muscle weakness caused by low-density lipoprotein receptor-related protein 4; MG caused by autoantibodies targeting LRP4 ligands; muscle weakness of unknown cause or whose target cannot be identified. Among them, more than 85% of patients with myasthenia gravis are caused by antibodies against AChR ...

AI Technical Summary

Problems solved by technology

Among the existing verification methods, pathological methods, such as observing muscle changes or autoimmune antibody levels through HE / immunohistochemical staining of muscle tissue sections, are currently recognized as the gold standard in the industry, but this method needs to be obtained by killing animals. For biological samples, the verification methods are not non-invasive, convenient and repeatable, and will cause irreversible damage to animals, making it impossible to continuously monitor and carry out follow-up research

The electromyography in the prior art uses low-frequency repetitive electrical stimulation to check the electrical attenuation response of muscles. Although it can reflect the changes in some muscles, this method mainly focuses on the neuromuscular junctions of the limbs, and the indicators are relatively single and cannot reflect The pathological changes of myocardium and gastrointestinal smooth muscle, let alone reveal the changes of immune multisystem muscles

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