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HER2 positive gastric cancer drug-resistant PDX model as well as construction method and application thereof

A drug-resistant and positive technology for gastric cancer, which can be used in animal husbandry and other directions to solve the problem of difficult access to tumor tissue.

Inactive Publication Date: 2022-01-21
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difficulty of obtaining tumor tissue after treatment, there is no successful establishment of a PDX model of gastric cancer resistant to HER2-positive trastuzumab after treatment

Method used

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  • HER2 positive gastric cancer drug-resistant PDX model as well as construction method and application thereof
  • HER2 positive gastric cancer drug-resistant PDX model as well as construction method and application thereof
  • HER2 positive gastric cancer drug-resistant PDX model as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] This example provides a HER2-positive gastric cancer drug-resistant PDX model, which is constructed by the following steps:

[0024] (1) Gastric cancer tissue from a patient with HER2-positive gastric cancer who is clinically drug-resistant after trastuzumab treatment was biopsied, and immunohistochemical detection was performed on the tissue of the gastric cancer patient (results as follows: figure 1 shown) and record the patient's medication information in detail;

[0025] (2) Cut the fresh gastric cancer tissue into 2×2×3mm 3 Small and large fragments were inoculated subcutaneously on the back of 4-6 week-old nude mice by bilateral subcutaneous inoculation. Tumor-forming mice such as figure 2 shown;

[0026] (3) Measure the body weight of nude mice at least twice a week (results such as image 3 shown) and observe and measure the transplanted gastric cancer tissue (the results are as follows Figure 4 shown), until the tumor grows to 1000-1500mm 3 size, taken o...

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Abstract

The invention relates to a construction method of an HER2 positive gastric cancer drug-resistant PDX model, which comprises the step of transplanting fresh gastric cancer tissues of a drug-resistant HER2 positive gastric cancer patient after drug treatment to the subcutaneous part of a mouse. The gastric cancer tissue of the drug-resistant patient treated by HER2 positive trastuzumab is clinically obtained, and the gastric cancer PDX model is successfully established. The scheme provided by the invention plays a crucial role in understanding a potential molecular mechanism of acquired drug resistance of trastuzumab, searching a new treatment strategy for overcoming drug resistance and formulating a next effective treatment scheme.

Description

technical field [0001] The present invention relates to a cancer treatment model and its application, in particular to a HER2-positive gastric cancer drug-resistant PDX model and its construction method and application. Background technique [0002] Gastric cancer is the fourth most common cancer and the second highest in cancer-related mortality. Although early diagnosis and treatment can reduce mortality, about 40% of patients are inoperable due to advanced stage or suffer from metastatic disease, the prognosis is poor, and palliative care is needed. Human epidermal growth factor receptor 2 (HER2, also known as ERBB2) is a transmembrane receptor tyrosine kinase whose overexpression / amplification (HER2 positive, HER2+) accounts for approximately 6.1%–23.0% of GCs. Several studies have confirmed the efficacy of trastuzumab in HER2+ metastatic gastric cancer. The ToGA study is the first clinical trial to show that trastuzumab combined with chemotherapy can significantly imp...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/0271A01K2207/12A01K2207/15A01K2227/105A01K2267/0331
Inventor 应杰儿尹露茜徐琦朱秀韦青徐晓青
Owner ZHEJIANG CANCER HOSPITAL
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