Construction method and application of plant gene expression vector

Abstract

The invention belongs to the technical field of biology, and discloses a construction method and application of a plant gene expression vector. The construction method of the plant gene expression vector comprises the following steps of: separating a gene sequence corresponding to a target gene from a plant genome, designing a polymerase chain reaction primer according to the target gene, and carrying out PCR, thereby obtaining a gene sequence corresponding to the target gene; constructing a plasmid vector, specifically, introducing enzyme cutting sites, and carrying out double enzyme cutting treatment, electrophoresis, gel recovery, connection, transformation and extraction on plasmids to obtain the plasmid vector; and connecting a target gene-containing object gene sequence to the constructed plasmid vector to construct a target gene-containing plant expression vector. With the construction method adopted, the gene expression vector can be rapidly constructed. The method is simple and convenient, low in cost and high in success rate. According to the gene expression vector constructed by the method disclosed by the invention, the inserted gene can be stably expressed in a plant body, and the gene expression vector can be used for screening and observing cells in a transgenosis process, so that the transgenosis efficiency is effectively improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a construction method and application of a plant gene expression vector. Background technique [0002] At present, the rapid development and wide application of plant genetic engineering technology has opened up broad prospects for the genetic improvement of plants. This technology overcomes the limitations of plant sexual hybridization, and can introduce genes from different species or even artificial synthesis into plants, thereby improving plant traits and cultivating new varieties of high-quality and high-yield crops. In the research and application of molecular biology such as gene cloning, gene function analysis, and gene transformation, vector construction is a commonly used technical means, and the use of appropriate expression vectors and construction methods will make the experimental effect twice the result with half the effort. However, the genes expressed by...

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Problems solved by technology

[0003] Through the above analysis, the problems and defects of the existing technology are: the gene expressed by the existing plant gene expression vector is unstable, and the construction success rate is low, the connection rate is not high, and the operation is complicated and expensive

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