Specific molecular marker primer and method for identifying 'Xianhu series' dendrobium candidum

A Dendrobium officinale, molecular marker technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. problem, to achieve the effect of high sensitivity and simple method

Pending Publication Date: 2022-01-28
ZHEJIANG SHOUXIANGU PHARMA CO LTD +2
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From ancient times to the present, the precious medicinal materials of the genus Dendrobium mainly come from natural wild resources, but the reproductive rate of wild resources is low, coupled with excessive excavation by humans, the wild resources of Dendrobium are now on the verge of extinction, resulting in unstable production and internal quality, which affects The quality of medicinal materials

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Specific molecular marker primer and method for identifying 'Xianhu series' dendrobium candidum
  • Specific molecular marker primer and method for identifying 'Xianhu series' dendrobium candidum
  • Specific molecular marker primer and method for identifying 'Xianhu series' dendrobium candidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Extraction of genomic DNA from Dendrobium officinale germplasm to be tested

[0030] Take 0.3-0.5 g of the germplasm of Dendrobium officinale to be tested, extract the genomic DNA of the germplasm of Dendrobium officinale to be tested by CTAB method, conduct electrophoresis on the obtained DNA with 1.5% agarose gel to check the purity, and use the Thermo Fisher Scientific Company The DNA concentration was detected by NanoDropTM 1000 ultra-micro UV spectrophotometer, then diluted to 50ng / μL, and placed in a 4-degree refrigerator for later use.

[0031] 2. Synthesize specific molecular marker primers

[0032] Molecular marker primers based on the specific sequence of Dendrobium officinale, upstream primer GLMI001-F: 5'-AACTGACATGCAAGAGTCGC-3' and downstream primer GLMI001-R: 5'-ATCTGCCAGCAAGGATGAGG-3', were synthesized by Shanghai Shenggong.

[0033] 3. PCR amplification of specific molecular marker primers (DK001-F / R)

[0034] PCR amplification system (total volume ...

Embodiment 2

[0040] Example 2: Stability test

[0041] According to the method of Example 1, the specific molecular marker DK001 was used to detect the "Dendrobium series" Dendrobium officinale germplasm of the same batch of 4 varieties (lines) with different repetitions. The results are shown in figure 2 , get consistent results:

[0042] Numbers 1 to 3 are individuals of Dendrobium candidum "Xianhu No. 1", and specific DNA bands between 250bp and 1500 bp are not amplified; those numbered 4 to 6 are individuals of Dendrobium candidum "Xianhu No. 2". At the same time, DNA bands of about 250bp and 1500bp were amplified; the numbered 7 to 9 were the individual Dendrobium officinale "Xianhu No. 3", and only one DNA band of about 1500bp was amplified; the numbered 10 to 12 were "Xianhu No. 4" Dendrobium officinale only amplified a DNA band of about 250bp.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a specific molecular marker primer for identifying 'Xianhu series' dendrobium candidum strains and a method for rapidly identifying the 'Xianhu series' dendrobium candidum strains by using the primer. The primer sequence is as follows: an upstream primer DK001-F: 5 '-AACTGACATGCAAGAGTCGC-3', and a downstream primer DK001-R: 5 '-ATCTGCCAGCAAGGATGAGG-3'. The specific molecular marker primer (DK001-F / R) disclosed by the invention has the beneficial effects that the Xianhu series dendrobium candidum can be quickly identified, the method is simple, whether a strip exists or not can be judged only through PCR (Polymerase Chain Reaction) and electrophoresis, and the specific molecular marker primer is accurate, efficient and high in sensitivity and is an irreplaceable molecular means for identifying the Xianhu series dendrobium candidum through apparent characteristics.

Description

(1) Technical field [0001] The invention relates to a specific molecular marker primer for identifying "Dendrobium series" Dendrobium officinale strains, and a method for rapidly identifying "Xianhu series" Dendrobium officinale strains by using the primers. (2) Background technology [0002] Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) is a perennial herb of the genus Dendrobium of the Orchidaceae family, also known as black knotweed, orchid. It grows on semi-shady mountain rocks above 1600 meters above sea level, and is mainly distributed in Zhejiang, Anhui, Fujian and other places in my country. Dendrobium candidum is a commonly used Chinese herbal medicine plant in my country. Li Shizhen recorded in the Compendium of Materia Medica: "Dendrobium can eliminate the qi, nourish the five viscera and lose weight, strengthen the yin and nourish the essence. Qi, grow muscles, dispel evil heat and prickly heat on the skin, pain in the feet and knees...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2565/125
Inventor 李振皓李明焱周莎莎熊晖蒋燕燕王晓彤陈美红
Owner ZHEJIANG SHOUXIANGU PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap