Organ-like chip and preparation method thereof
A technology of organ chips and types, applied in the field of organ culture, can solve the problems of few types of organs, difficult cleaning, unfavorable miniaturization and integration, etc., and achieve the effect of convenient operation
Pending Publication Date: 2022-02-08
北京龙迈达斯科技开发有限公司
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- Application Information
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Problems solved by technology
[0004] The upper and lower layer cells of the existing organoid chips overlap, and there are fewer types of organs to be cultured, which is not conducive to miniaturization and integration, and it is difficult to clean
Method used
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Embodiment Construction
[0042] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.
[0043] Please refer to Figure 1-4 , the present invention provides an organoid chip, comprising a chip box body 50, the chip box body 50 is sequentially provided with a first layer 10 for culturing cells, a second layer 20 for barrier function, and a layer for material exchange. a third layer 30 and a fourth layer 40 for culturing cells;
[0044] The first layer 10 includes a first base plate 101, on which a plurality of groups of first culture areas 102 are arranged, and the first culture areas 102 are provided with first cell culture chambers 105 and second cell culture chambers 106 arranged in sequence and communicated with each other. , the third cell culture chamber 107 and the fourth cell culture chamber 108, and the fifth cell culture cha...
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The invention discloses an organ-like chip and a preparation method thereof, and belongs to the technical field of organ culture. The organ-like chip comprises a chip box body, and the chip box body is sequentially provided with a first layer used for culturing cells, a second layer playing a blocking role, a third layer used for substance exchange and a fourth layer used for culturing cells from top to bottom; the first layer comprises a first substrate, and a plurality of groups of first culture areas are arranged on the first substrate; and the first culture area is provided with a first cell culture chamber, a second cell culture chamber, a third cell culture chamber and a fourth cell culture chamber which are sequentially arranged and communicate with each other, and is provided with a fifth cell culture chamber, a sixth cell culture chamber, a seventh cell culture chamber and an eighth cell culture chamber which are sequentially arranged and communicate with each other. According to the organ-like chip and the preparation method thereof provided by the invention, various cells can be cultured, miniaturization and integration are facilitated, and the organ-like chip is easy to clean.
Description
technical field [0001] The invention relates to an organoid chip and a preparation method thereof, belonging to the technical field of organ culture. Background technique [0002] With the development of cell biology and organoid technology, three-dimensional cell culture technology is gradually replacing traditional two-dimensional cell culture technology. At present, various types of cells have strong self-assembly ability, such as pluripotent stem cells, tumor cells, tissue cells, etc. Three-dimensional cell spheroids are three-dimensional aggregates formed by the self-assembly of various cells, which are closer to the structure and morphology of tissue cells in vivo and are more conducive to the study of their functional mechanisms. [0003] The organ chip is a three-dimensional cell culture system based on a multi-channel fluid chip, which is composed of multiple cell culture partitions that simulate the environment of human tissues and organs. Connections are made in...
Claims
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Patent Timeline
Login to View More IPC IPC(8): C12M3/00C12M3/06C12M1/34
CPCC12M21/08C12M23/38C12M23/48C12M29/04C12M35/08C12M41/32
Inventor 毛静涛孙飞飞
Owner 北京龙迈达斯科技开发有限公司